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Click Chemistry Tools

Chemical diversity is just a click away

baseclick GmbH

Click chemistry tools for simple labeling

You have no or little experience with click chemistry, but you need to perform click reactions? No problem. We are offering reagents, kits and protocols for your click reactions. We want to help you to experience a new world of simple and reliable modification at affordable cost. You will see that click chemistry can be used by everyone to generate chemical diversity for innovative applications.

You are already using click chemistry and are searching for a reliable compound provider? Then you are also right at baseclick.

The key attributes of baseclick’s click reagents and tools are:

  • Tested click performance for good results help to save your time
  • Click labeling at affordable cost to save your money
  • Highly reliable and efficient labeling
  • Big range of molecules to select from for labelling, to allow flexibility
  • You need another label? We will try to get it.

Applications

Baseclick’s click reagents and tools offer full labeling flexibility for your experiment. Due to the high labeling efficiency and the modularity of the technology, they can be used to e.g. create novel detection applications or prepare biomolecules with augmented functionality. Of course, we also offer reliable reagents and tools to perform click reactions.

Which reagent is the right for me?

Fluorescent labels are usually the first choice for detection applications. For metabolic labeling modified metabolites can be fed to cells, e. g. nucleosides, sugars, fatty acids and amino acids. In order to catalyze click reactions, we offer ready-to-use reagents and even kits to simplify your workflow.

You have no idea how to perform click reactions? Don’t worry we have standard click protocols to help you get started.

Literature

Click Reagents & Tools / Click Chemistry

  • Discovery of the copper-catalyzed click conditions[1,2]
  • The role and effect of copper(I)-stabilizing ligands[3–5]
  • Click chemistry on oligonucleotides for labeling[6,7]
  • Click chemistry compounds as tools for biological applications[8]

Selected references

[1] V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chemie – Int. Ed. 2002, 41, 2596–2599.
[2] C. W. Tornøe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057–64.
[3] Q. Wang, T. R. Chan, R. Hilgraf, V. V. Fokin, K. B. Sharpless, M. G. Finn, J. Am. Chem. Soc. 2003, 125, 3192–3193.
[4] T. R. Chan, R. Hilgraf, K. B. Sharpless, V. V Fokin, Org. Lett. 2004, 6, 2853–2855.
[5] C. Besanceney-Webler, H. Jiang, T. Zheng, L. Feng, D. Soriano Del Amo, W. Wang, L. M. Klivansky, F. L. Marlow, Y. Liu, P. Wu, Angew. Chemie – Int. Ed. 2011, 50, 8051–8056.
[6] J. Gierlich, G. a. Burley, P. M. E. Gramlich, D. M. Hammond, T. Carell, Org. Lett. 2006, 8, 3639–3642.
[7] J. Gierlich, K. Gutsmiedl, P. M. E. E. Gramlich, A. Schmidt, G. A. Burley, T. Carell, Chem. – A Eur. J. 2007, 13, 9486–9494.
[8] M. Grammel, H. C. Hang, Nat. Chem. Biol. 2013, 9, 475–484.

FAQs

  • My CuBr-click solution turns brownish. Can I still use it?

    Good CuBr should result in a yellow-greenish to green clear solution upon dissolving. If your catalyst turns brownish upon addition of the click solution it is most likely degraded (oxidized). Please use a fresh batch of CuBr to ensure optimal click reaction catalysis.

  • What is the yield of click reactions?

    The nature of click chemistry is that the reactions are fast, efficient and give good yields. Under optimal conditions, quantitative yields can be achieved easily. High concentrated or low concentrated samples are usually more difficult to optimize, but should still give yields of more than 50-80%.

  • Are there reagents to avoid in a copper catalyzed click reaction?

    Some common biochemical buffer components can interfere with the progress of copper-catalyzed click reactions (CuAAC) and therefore should be avoided. For example, TE and TAE buffer contain EDTA which chelates the catalytic copper ions and thus decreases reaction kinetic and yield. Moreover, free thiols, like dithiothreitol (DTT) and -mercapto ethanol, can deteriorate or completely stop click reactions.

  • What kind of labels can be clicked?

    As it is the nature of click reactions, various groups can be attached to nucleic acids, not constricted to dyes. The only requirement is that you are using high quality reagents for your reactions and each reaction partner is soluble. Baseclick therefore provides a wide range of clickable groups, linkers and fluorescent dyes, fatty acids, etc. all tested in click reactions.

  • What is the best catalyst for my reaction?

    The optimal catalyst depends on your application and the solvents used. In general, there are three major catalyst systems for copper-catalyzed click reactions that apply the required Cu(I) source:

    1) You are working in aqueous media: Then we recommend using a CuSO4 (BCMI-004) based system. To generate the catalytic Cu(I) species, please do not forget to add the necessary sodium ascorbate (BCMI-005) and our water soluble THPTA ligand (BCMI-006). As the sodium ascorbate has restricted stability when dissolved, please prepare this solution always fresh.

    2) When your medium is of organic nature (mostly DMSO or click solution) then use CuBr and our TBTA ligand (BCMI-002) for labeling reactions. We would like to note, that CuBr is sensitive to air. Therefore, we take precautions when aliquoting and sealing this compound. Once opened, please use the solution fast (within a few hours maximum).

    3) For small sample amounts from e.g. enzymatic reactions in aqueous solvents we recommend using our reactor system that is part of our Oligo Click kits. As this catalyst will not dissolve during the reaction and can also be applied in organic media, its handling is very simple as you will see in the kits user manual. Additionally, the long-term storage is superior compared to the other catalyst systems.

  • What does baseclick mean by shelf life?

    The shelf life of each product is stated on its data sheet. This information means the period after receipt of an order for which we guarantee the specified quality on this product. If the product is stored as directed, the product will meet these specifications at the first opening and usage. Upon appropriate handling of the compound, this should be valid also for subsequent usages.

    Anyhow, outside of baseclick each product will face different conditions (e.g. inert atmosphere, evaporation, light exposure, moisture), which can influence the product´s fitness once opened and stored in your hands for some time. Therefore, we cannot guarantee the same specifications after opening and extended storage.

  • What are the Eterneon dyes?

    Our EterneonTM dyes are a special class of ultra-stable fluorescent dyes. Our long stokes-shift ones even have well separated peaks of absorbance and emission, so it is very convenient to characterize your sample with very sharp and strongly fluorescent signals. These special dyes are oligothiophene based, uncharged and not pH sensitive.