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New patent application


For more than ten years, baseclick GmbH has been providing targeted modification of nucleic acids mainly in detection-based applications with its technology, research and products. These can be used for the detection of gene mutations, presence of gene sequences or cell proliferation.
 
Recently, innovation drivers in the field of "Next Generation Sequencing" became aware of the baseclick technology and apply it very successfully, e.g. in single cell sequencing.
While the sequencing itself is already well advanced and the determination of the base sequencing is possible with a high precision, the sample preparation still shows some difficulties. These problems were mainly demonstrated in cumbersome, time-consuming and sometimes unspecific protocols. The aim of the sample preparation is mainly to modify the probing material with so-called "adapters", which are required for deep sequencing.
 
With its unique bioconjugation technology, the "click-chemistry", baseclick GmbH was now successful by combining two nucleic acid molecules (sequence and "adapter") efficiently, site-specifically and compatible with further PCR amplification via chemical non-enzymatic ligation.

In this way it is now possible to waive the previously required second strand synthesis in RNA sequencing. Of particular interest to this invention are the so-called "backbone mimics". These are artificial alternatives to the natural phosphodiester bond that can be generated by copper-catalyzed azide-alkyne cycloadditions (CuAAC). By using PCR in model systems, these bonds could be converted into copies with their natural phosphodiester backbones without a mutation of the sequence and are thus completely biocompatible.
"This click conjugation method has shown one more time its strengths and will help to make deep sequencing a more robust, versatile, and cost-effective method of diagnostics in the future," a comment on the significance of the new invention by Dr. Thomas Frischmuth, CEO of baseclick GmbH.

In addition to the patent application of the method (EP 2017/17194093 (Click based ligation)), the company plans to place a corresponding kit on the market by the beginning of 2019.

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