Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Catalog No.
- 1.0 µmol
- 250 €
You can use our amine-triphosphates in your nucleotide mixture and label your PCR-fragments subsequently with NHS-ester markers. The incorporation of aminoallyl-(d)NTPs is accepted by many polymerases in PCR (e.g. 5-Aminoallyl-dUTP has been enzymatically incorporated into DNA using Taq DNA polymerase, Reverse Transcriptases, phi29 DNA Polymerase, Klenow Fragment, exo- and DNA Polymerase I).
Aminoallyl-NTPs are used e.g. for the synthesis of labeled fluorescent probes by reverse transcription of RNA
Chem. Eur. J., 2007, 13, 9486-9494.
J. Am. Chem. Soc., 2006, 128, 1398-1399.
12 months unopened after receipt
≥ 90% (HPLC)
100 mM solution in water
λmax = 290 nm
- Molecular Formula