We enable nucleic acid labeling bioconjugation
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AA-dCTP (Aminoallyl-dCTP)

Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1.0 µmol
  • BCT-09-S

  • € 130,00
  • 10 µmol
  • BCT-09-L

  • € 680,00
Clear
  • You can use our amine-triphosphates in your nucleotide mixture and label your PCR-fragments subsequently with NHS-ester markers. The incorporation of aminoallyl-dNTPs is accepted by many polymerases in PCR (e.g. 5-Aminoallyl-dUTP has been enzymatically incorporated into DNA using Taq DNA polymerase, Reverse Transcriptases, phi29 DNA Polymerase, Klenow Fragment, exo- and DNA Polymerase I).
    Aminoallyl-dNTPs have shown to act as stable nucleotide for the synthesis of complementary DNA (cDNA) synthesis or the immunoprecipitation probes.

    LITERATURE

    Chem. Eur. J., 2007, 13, 9486-9494

    J. AW. Chem. Soc., 2006, 128, 1398-1399

    • Molecular Formula

      C12H21N4O13P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      522.00 g/ mol

    • Purity

      ≥ 95 % (HPLC)

    • Physical State

      100 mM solution in water

    • CAS Number

      n.a.

    • Absorption (max)

      λmax = 291 nm

    • Ɛ (max)

      5,050 cm-1M-1

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