We enable nucleic acid labeling bioconjugation
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AA-dUTP (Aminoallyl-dUTP)

Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1.0 µmol
  • BCT-10-S

  • € 130,00
  • 10 µmol
  • BCT-10-L

  • € 680,00
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  • You can use our amine-triphosphates in your nucleotide mixture and label your PCR-fragments subsequently with NHS-ester markers. The incorporation of aminoallyl-dNTPs is accepted by many polymerases in PCR (e.g. 5-Aminoallyl-dUTP has been enzymatically incorporated into DNA using Taq DNA polymerase, Reverse Transcriptases, phi29 DNA Polymerase, Klenow Fragment, exo- and DNA Polymerase I).
    Aminoallyl-dNTPs have shown to act as stable nucleotide for the synthesis of complementary DNA (cDNA) synthesis or the immunoprecipitation probes.

    LITERATURE

    C. C. Xiang, O. A. Kozhich, M. Chen, J. M. Inman, Q. N. Phan, Y. Chen, M. J. Brownstein, Net. Biotechnol., 2002, 20, 738-742

    Dirsch et al., Appl. Immohistochem. Mol. Morphol., 2007, 3:332

    Cox et al., BioTechniques, 2004, 36:114

    • Molecular Formula

      C12H20N3O14P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      523.22 g/mol

    • Purity

      ≥ 95 % (HPLC)

    • Physical State

      100 mM solution in water

    • CAS Number

      n.a.

    • Absorption (max)

      λmax = 291 nm

    • Ɛ (max)

      7,100 cm-1M-1

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