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AA-dUTP (Aminoallyl-dUTP)
Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
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You can use our amine-triphosphates in your nucleotide mixture and label your PCR-fragments subsequently with NHS-ester markers. The incorporation of aminoallyl-dNTPs is accepted by many polymerases in PCR (e.g. 5-Aminoallyl-dUTP has been enzymatically incorporated into DNA using Taq DNA polymerase, Reverse Transcriptases, phi29 DNA Polymerase, Klenow Fragment, exo- and DNA Polymerase I).
Aminoallyl-dNTPs have shown to act as stable nucleotide for the synthesis of complementary DNA (cDNA) synthesis or the immunoprecipitation probes.LITERATURE
C. C. Xiang, O. A. Kozhich, M. Chen, J. M. Inman, Q. N. Phan, Y. Chen, M. J. Brownstein, Net. Biotechnol., 2002, 20, 738-742
Dirsch et al., Appl. Immohistochem. Mol. Morphol., 2007, 3:332
Cox et al., BioTechniques, 2004, 36:114
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Molecular Formula
C12H20N3O14P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
523.22 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
100 mM solution in water
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CAS Number
1796578-18-1 (trisodium salt)
264270-80-6 (tetrasodium salt)
116840-18-7 (free acid)
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Absorption (max)
λmax = 291 nm
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Ɛ (max)
7,100 cm-1M-1
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Molecular Formula