Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Catalog No.
- 1.0 µmol
- € 130,00
- 10 µmol
- € 680,00
You can use our amine-triphosphates in your nucleotide mixture and label your PCR-fragments subsequently with NHS-ester markers. The incorporation of aminoallyl-dNTPs is accepted by many polymerases in PCR (e.g. 5-Aminoallyl-dUTP has been enzymatically incorporated into DNA using Taq DNA polymerase, Reverse Transcriptases, phi29 DNA Polymerase, Klenow Fragment, exo- and DNA Polymerase I).
Aminoallyl-dNTPs have shown to act as stable nucleotide for the synthesis of complementary DNA (cDNA) synthesis or the immunoprecipitation probes.
C. C. Xiang, O. A. Kozhich, M. Chen, J. M. Inman, Q. N. Phan, Y. Chen, M. J. Brownstein, Net. Biotechnol., 2002, 20, 738-742
Dirsch et al., Appl. Immohistochem. Mol. Morphol., 2007, 3:332
Cox et al., BioTechniques, 2004, 36:114
12 months unopened after receipt
≥ 95 % (HPLC)
100 mM solution in water
λmax = 291 nm
- Molecular Formula