C8-TIPS-dC-CEP

This phosphoramidite is used under standard conditions for solid phase synthesis of oligonucleotides. It has shown to be incorporated into DNA oligomers with good coupling efficiency. The protection group on the alkyne can be exploited to introduce different labels by click chemistry into one oligo. Having reacted a first group and purified the compound, the TIPS can then be deprotected and is thus ready for a second click reaction with another click azide on the same product.

LITERATURE

P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, Angew. Chemie – Int. Ed. 2008, 47, 3442–3444.

P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chemie – Int. Ed. 2008, 47, 8350–8358.