ClickTech Library Kit full-length mRNA_Seq

Baseclick’s ClickTech Library Kit full-length mRNA_Seq has been developed as method for e.g. sequencing of total mRNA or the whole exome of cells for genetic diagnosis. The kit provides reagents for cDNA synthesis of any RNA pool and introduces a single azide nucleotide at the 3’END of cDNA. This azide modified cDNA can react with an alkyne-adapter sequence in a highly selective fashion under benign click reaction conditions. With the included special designed Primer, the adapter clicked cDNA can be used for PCR amplification of the cDNA pool as such or for targeted genes amplification.

BCK-CTL-FRS_10: If you use the ClickTech Library Kit full-length mRNA_Seq from baseclick without the recommended DNA BaseClean Kit you must follow the requirements of your purification kit supplier. Furthermore, please notice that the results may differ from the expectations as described in this user manual. In addition to that, in some cases it might be helpful to read section 10 “Trouble Shooting” to avoid typical application issues with other purification kits.

BCK-CTL-FRS+P_10: Our DNA BaseClean Kit for purification steps is included.