ClickTech Library Kit full-length mRNA_Seq

Baseclick’s ClickTech Library Kit full-length mRNA_Seq has been developed as method for e.g. sequencing of total mRNA or the whole exome of cells for genetic diagnosis. The kit provides reagents for cDNA synthesis of any RNA pool and introduces a single azide nucleotide at the 3’END of cDNA. This azide modified cDNA can react with an alkyne-adapter sequence in a highly selective fashion under benign click reaction conditions. With the included special designed Primer, the adapter clicked cDNA can be used for PCR amplification of the cDNA pool as such or for targeted genes amplification.