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Eterneon²488 Azide (FITC alternative)
Ultra sensitive Fluorescent Dye to label DNA/RNA
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This special green-fluorescent dye exhibits advanced click reaction kinetics. Therefore, also less amount of copper can be used in the reaction mixture without any effect on the great reaction efficiency. This effect can be crucial e.g. when copper sensitive enzyme or biomolecules shall be labeled. Once attached to a biomolecule fluorescence microscopy detection can be carried out just like with FAM, FITC, or any other alternative dye.
LITERATURE
L. Liang, J. Li, Q. Li, Q. Huang, J. Shi, H. Yan, C. Fan, Angew. Chemie – Int. Ed. 2014, 53, 7745–7750.
FAQ
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What are the Eterneon dyes?
Our EterneonTM dyes are a special class of ultra-stable fluorescent dyes. Our long stokes-shift ones even have well separated peaks of absorbance and emission, so it is very convenient to characterize your sample with very sharp and strongly fluorescent signals. These special dyes are oligothiophene based, uncharged and not pH sensitive.
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What are the Eterneon dyes?
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Molecular Formula
C30H22N6O7
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
580.55 g/mol
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Purity
≥ 90 % (HPLC)
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Physical State
yellow to orange solid
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CAS Number
n.a.
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Additional name
FITC alternative; Alexa Fluor 488 alternative; CF Dye 488 alternative; DyLight 488 alternative
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Excitation (max)
PBS; pH 7.5: 490 nm
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Emission (max)
PBS, pH 7.5: 510 nm
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Ɛ (max)
PBS, pH 7.5: 76,000 cm-1M-1
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Solubility
DMSO, DMF
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Preparation/Handling
For a 10 mM solution add 172 μL to 1 mg.
For a 10 mM solution add 861 μL to 5 mg.
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Molecular Formula