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Eterneon²488 Azide (FITC alternative)
Fluorescent dye to label DNA/RNA
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This special green-fluorescent dye enables advanced click reaction kinetics. Therefore, less amount of copper can be used achieving the same reaction efficiency. This effect can be crucial e.g. when copper sensitive enzyme or biomolecules shall be labeled. Once clicked to a biomolecule fluorescence detection is identical to FAM, FITC, or any other alternative dye.
LITERATURE
Single-Particle Tracking and Modulation of Cell Entry Pathways of a Tetrahedral DNA Nanostructure in Live Cells, L. Liang et al., 2014, Angew. Chemie Int. Ed., Vol. 53, p. 7745–7750.
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Molecular Formula
C30H22N6O7
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
580.55 g/mol
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Purity
≥ 90% (HPLC)
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Physical State
yellow to orange solid
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CAS Number
n.a.
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Additional name
FITC alternative; Alexa Fluor 488 alternative; CF Dye 488 alternative; DyLight 488 alternative
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Excitation (max)
PBS; pH 7.5: 490 nm
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Emission (max)
PBS, pH 7.5: 510 nm
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Ɛ (max)
PBS, pH 7.5: 76,000 cm-1M-1
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Solubility
DMSO, DMF
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Preparation/Handling
For a 10 mM solution add 172 μL to 1 mg.
For a 10 mM solution add 861 μL to 5 mg.
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Molecular Formula