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Eterneon² 649 Azide (Cy5TM alternative)
Ultra sensitive Fluorescent Dye to label DNA/RNA
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This special red fluorescent dye exhibits advanced click reaction kinetics. Therefore, also less amount of copper can be used in the reaction mixture without any effect on the great reaction efficiency. This effect can be crucial e.g. when copper sensitive enzyme or biomolecules shall be labeled. Once attached to a biomolecule fluorescence microscopy detection can be carried out just like with Cy5, or any other alternative dye.
LITERATURE
H. Jiang et al, Bioconjugate Chem., 2014, 25, 698-706
C. Uttamapinant, et al., Angew. Chem. Int. Ed., 2012, 51, 5852-56
A. Geabler et al., J- Lipid. Res., 2016, 57, 1934-47
FAQ
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What are the Eterneon dyes?
Our EterneonTM dyes are a special class of ultra-stable fluorescent dyes. Our long stokes-shift ones even have well separated peaks of absorbance and emission, so it is very convenient to characterize your sample with very sharp and strongly fluorescent signals. These special dyes are oligothiophene based, uncharged and not pH sensitive.
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What are the Eterneon dyes?
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Molecular Formula
C43H52N8O11S3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
953.15 g/mol
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Purity
> 90 % (HPLC)
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Physical State
blue solid
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CAS Number
n.a.
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Additional name
Cy5TM alternative; Alexa Fluor 647 alternative; CF Dye 647 alternative; DyLight 649 alternative
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Absorption (max)
PBS; pH 7.5: 647 nm
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Emission (max)
PBS; pH 7.5: 663 nm
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Ɛ (max)
PBS; pH 7.5: 225,000 cm-1M-1
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Solubility
Water, DMSO, DMF, MeOH, EtOH
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Preparation/Handling
For a 10 mM solution add 104 μL to 1 mg.
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Molecular Formula