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Eterneon²Biotin Azide (Biotin analogue)
Biotinylation Reagent for Labeling DNA/RNA
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This special biotinylation reagent exhibits advanced click reaction kinetics. Therefore, also less amount of copper can be used in the reaction mixture without any effect on the great reaction efficiency. This effect can be crucial e.g. when copper sensitive enzyme or biomolecules shall be labeled. Once attached to a biomolecule fluorescence microscopy detection can be carried out using the well-known signal enhancement strategy of incubation with a fluorescent streptavidin-conjugate.
LITERATURE
H. Jiang et al, Bioconjugate Chem., 2014, 25, 698-706
C. Uttamapinant, et al., Angew. Chem. Int. Ed., 2012, 51, 5852-56
A. Geabler et al., J- Lipid. Res., 2016, 57, 1934-47
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Molecular Formula
C27H42N8O7S
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
622.74 g/mol
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Purity
≥ 93 % (HPLC)
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Physical State
off-white to slightly orange/yellow
amorpheus solid -
CAS Number
n.a.
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Additional name
Biotin analogue
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Solubility
DMSO, DMF, MeOH
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Preparation/Handling
For a 10 mM solution add 160 μL to 1mg.
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Molecular Formula