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nCoV qPCR Primers & Probes N1

Primers and probes recommended by the CDC for SARS-CoV-2 detection

Currency:  
  • Size
  • Catalog No.
  • Price
  • 20µL (Forward 20µM 50 PCR)
  • BCC-19nCoVN1_F50

  • € 3,00
  • 20µL (Reverse 20µM 50 PCR)
  • BCC-19nCoVN1_R50

  • € 3,00
  • 20µL (Probe 5µM 50 PCR)
  • BCC-19nCoVN1_P50

  • € 3,00
  • 3 x 20µL (Set 50 PCR)
  • BCC-19nCoVN1_S50

  • € 6,00
  • 40µL (Forward 20µM 100 PCR)
  • BCC-19nCoVN1_F100

  • € 4,00
  • 40µL (Reverse 20µM 100 PCR)
  • BCC-19nCoVN1_R100

  • € 4,00
  • 40µL (Probe 5µM 100 PCR)
  • BCC-19nCoVN1_P100

  • € 4,00
  • 3 x 40µL (Set 100 PCR)
  • BCC-19nCoVN1_S100

  • € 8,00
Clear
  • Baseclick provide primers and probes recommended by the Centers for Disease Control and Prevention (CDC) to researchers for the in vitro detection of the novel coronavirus (2019-nCoV).

    The primers and probes are designed for the universal detection of SARS-like coronaviruses (N3 assay) and for the specific detection of novel 2019-nCoV (N1 and N2 assays).

    RP – all clinical samples should be tested for human RNAse P (RNP) gene to assess specimen quality.

    You are intersted in primers and probes to create a Real-Time RT-PCR panel in your lab, following the panel instructions from the CDC to get more instructions for use.

    Catalog No. Description Sequence (5’-3’) Lenght Label.
    BCC-19nCoVN1_F50 Forward Primer for 50 qPCR reactions GAC CCC AAA ATC AGC GAA AT 20 n.r.
    BCC-19nCoVN1_R50 Reverse Primer for 50 qPCR reactions TCT GGT TAC TGC CAG TTG AAT CTG 24 n.r.
    BCC-19nCoVN1_P50 Probe for 50 qPCR reactions FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1 24 FAM / BHQ1
    BCC-19nCoVN1_S50 Set of N1 Primers &Probe for 50 qPCR reactions GAC CCC AAA ATC AGC GAA AT
    TCT GGT TAC TGC CAG TTG AAT CTG
    FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1
    20
    24
    24
    n.r.
    n.r.
    FAM / BHQ1
    BCC-19nCoVN1_F100 Forward Primer for 100 qPCR reactions GAC CCC AAA ATC AGC GAA AT 20 n.r.
    BCC-19nCoVN1_R100 Reverse Primer for 100 qPCR reactions TCT GGT TAC TGC CAG TTG AAT CTG 24 n.r.
    BCC-19nCoVN1_P100 Probe for 100 qPCR reactions FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1 24 FAM / BHQ1
    BCC-19nCoVN1_S100 Set of N1 Primers &Probe for 100 qPCR reactions GAC CCC AAA ATC AGC GAA AT
    TCT GGT TAC TGC CAG TTG AAT CTG
    FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1
    20
    24
    24
    n.r.
    n.r.
    FAM / BHQ1

    These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations.

    FAQ

    • How can I extract the viral RNA form the specimens?

      The quality of the assay is largely dependent on the quality of input RNA.

      Commercially available extraction procedures that have been shown to generate highly purified RNA when following manufacturer’s recommended procedures for sample extraction include:

      -QIAamp® Viral RNA Mini Kit,

      -QIAamp® MinElute Virus Spin Kit or RNeasy® Mini Kit (QIAGEN),

      -EZ1 DSP Virus Kit (QIAGEN),

      -Roche MagNA Pure Compact RNA Isolation Kit,

    • What are the precaution I need to use?

      Wear appropriate personal protective equipment (e.g. gloves, eye protection) when working with clinical specimens. Specimens processing should be performed in a certified class II biological safety cabinet following biosafety level 2 guidelines.

    • What kind of specimens can I se for the assay?

      Respiratory specimens and sera, e.g. nasopharyngeal aspirates or washes, swabs, bronchioalveolar lavage and sputum can be used for the detection of virus RNA.

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Solubility

      water, aqueous buffer

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