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RNA Labeling Kit – 555
Kit to generate high labeled RNA samples
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The RNA Labeling Kit produces alkyne-labeled RNA by in vitro transcription from bacteriophage T7 RNA polymerase promoters. The kit utilizes 5-Ethynyl-UTP (5-EUTP) nucleotide to firstly decorate the RNA with alkynes during enzymatic sznthesis. The resulting RNA contains alkyne modified uracils, which subsequently react with azido-derivates of e.g. fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. One adevantage is that the label incorporation can be tuned by the amount of marker azide used in the click reaction.
FAQ
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What is the best way to handle RNA to avoid degradation?
Due to its chemical structure RNA is less stable than DNA. At high temperatures, under basic conditions (pH>9) and in the presence of high Mg2+ concentrations, chemical reactions that favour hydrolysis of the RNA molecule take place. Additionally, RNases are common contaminations in standard laboratory conditions compared to DNases. We recommend to clean the benches and equipment with RNase inhibitors or specific detergent mixtures and to wear fresh gloves when handling the tubes (gloves that have been worn or that e.g. touched door/refrigerator handles are considered contaminated). Moreover, RNase-free H2O and tubes should be used for buffer preparation and to dissolve the samples.
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What is the best way to store RNA to avoid degradation?
For storage we recommend to store RNA at –80 °C, which should conserve RNA for one year without significant degradation. Alternatively.
In our experience, short synthetic RNAs (up to 50mer) are more stable than long single-stranded RNAs from biological samples and therefore can be stored at –20 °C in a dry format. -
What kits are available from baseclick for RNA modification?
We offer several kits to prepare labelled RNAs that are based on enzymatic incorporation of alkyne- or azide-modified nucleotides and subsequent click labelling.
The RNA Labeling Kit allows you to introduce several alkyne groups randomly in your RNA through the incorporation of the 5-ethynyl-UTP (EUTP) by the T7 RNA polymerase during in vitro transcription.
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What is the best way to handle RNA to avoid degradation?
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Shelf Life
12 months unopened after receipt
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Storage Conditions
– 20 °C
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Physical State
kit system made of different components
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CAS Number
n.a.
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Excitation (max)
546 nm
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Emission (max)
579 nm
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Ɛ (max)
91.000 cm-1M-1
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Preparation/Handling
please see user manual of the kit
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Shelf Life