RNA Labeling Kit – 555
Kit to generate highly labeled RNA probes
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The RNA Labeling Kit produces alkyne-modified RNA by in vitro transcription from bacteriophage T7 RNA polymerase promoters. The kit utilizes 5-Ethynyl-UTP (5-EUTP) nucleotide to firstly incorporate alkynes into RNA. The resulting RNA contains alkyne modified Uracil, which subsequently react with azides of e.g. fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. One advantage is that the label incorporation can be tuned by the UTP to 5-EUTP ratio.
FAQ
- What is the best way to handle RNA to avoid degradation?
Due to its chemical structure RNA is less stable than DNA. At high temperatures, under basic conditions (pH>9) or in the presence of high Mg2+ concentrations, hydrolysis of RNA takes place. Additionally, RNases are common contaminations in standard laboratory conditions compared to DNases. We recommend to clean the benches and equipment with specific detergent mixtures and to wear fresh gloves when handling the tubes (gloves that have been worn or that e.g. touched door/refrigerator handles are considered contaminated). Moreover, RNase-free H2O and tubes should be used for buffer preparation and to dissolve the samples.
- What is the best way to store RNA to avoid degradation?
For storage we recommend to store RNA at –80 °C, which should conserve RNA for one year without significant degradation.
In our experience, short synthetic RNAs (up to 50mer) are more stable than long single-stranded RNAs from biological samples and therefore can be stored at –20 °C in solid state. - What kits are available from baseclick for RNA modification?
The RNA Labeling Kit allows you to introduce several alkyne groups randomly in your RNA through the incorporation of the 5-Ethynyl-UTP (5-EUTP) by the T7 RNA polymerase during in vitro transcription.
- What is the best way to handle RNA to avoid degradation?
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- Shelf Life
12 months unopened after receipt
- Storage Conditions
– 20 °C
- Physical State
kit system made of different components
- CAS Number
n.a.
- Excitation (max)
546 nm
- Emission (max)
579 nm
- Ɛ (max)
91.000 cm-1M-1
- Preparation/Handling
please see user manual of the kit
- Shelf Life