mRNA Configurator

Welcome to our mRNA Configurator section!

Baseclick is guiding provider of in vitro mRNA synthesis and modification service for research application. Click technology holds the promise to revolutionize the mRNA Technology in drug development, Cancer treatment and vaccine discovery and enables taliored (IVT) mRNA with superior quality. Our team of experts transforms your mRNA design to a developed product.

baseclick supplies diverse types of mRNA, we have splitted up our service in three categories:

RNA Name

Template Source

DNA Length

RNA Length

Template Type

Please selectcircular plasmidlinearized plasmid (no digestion required)PCR product

To proceed with your request, it is required that your template contains a T7 promoter site. Please provide the sequence of the three bases immediately after the T7 promoter:

Full Sequence

Upload a file (Textfile (txt, Word), FASTA)

5’ Capping

Please selectNo capping Anti-Reverse CAP-Analogue (ARCA) Clean-Cap AG Other:

CleanCap AG requires the initiation sequence 5’ AGG 3’ ARCA Cap Analog requires the initiation sequence 5’ GG 3’

Phosphatase Treatment

Please selectYesNo

Uracil Modifications

Please selectUnmodified Pseudouridine N1-Methylpseudouridine Other: 

Poly(A) Tail

Please selectNo tail (RNA) Average length 20-150 Nt Included in the template 

Enzyme for Linearization

Buffer

Please select1 mM Sodium Citrate, pH 6.4 5 mM TRIS, pH 7.5 Other: 

3’ Modification via 3’-Azido-ddATP

Please selectYesNo

Click Conjugation at 3’end

Please selectDBCO-PEG4-5/6-FAMDBCO-PEG4-5/6-TAMRACy5-DBCOManα1-6(Manα1-3)Manα-Phenyl-PEG5-DBCOTris-GalNAc-β-Ala-PEG4-DBCONoOther:

Yield

Please select50 µg100 µg500 µg

QC

Purity by A260/A280 ratio and Integrity by denaturing Agarose Gel

Report

+ Add another Transcript

RNA Name

Template Source

DNA Length

RNA Length

Template Type

Please selectcircular plasmidlinearized plasmid (no digestion required)PCR product

To proceed with your request, it is required that your template contains a T7 promoter site. Please provide the sequence of the three bases immediately after the T7 promoter:

Full Sequence

Upload a file (Textfile (txt,Word), FASTA, GeneBank)

5’ Capping

Please selectNo capping Anti-Reverse CAP-Analogue (ARCA) Clean-Cap AG Other:

CleanCap AG requires the initiation sequence 5’ AGG 3’ ARCA Cap Analog requires the initiation sequence 5’ GG 3’

Phosphatase Treatment

Please selectYesNo

Uracil Modifications

Please selectUnmodified Pseudouridine N1-Methylpseudouridine Other: 

Poly(A) Tail

Please selectNo tail (RNA) Average length 20-150 Nt Included in the template 

Enzyme for Linearization

Buffer

Please select1 mM Sodium Citrate, pH 6.4 5 mM TRIS, pH 7.5 Other: 

3’ Modification via 3’-Azido-ddATP

Please selectYesNo

Click Conjugation at 3’end

Please selectDBCO-PEG4-5/6-FAMDBCO-PEG4-5/6-TAMRACy5-DBCOManα1-6(Manα1-3)Manα-Phenyl-PEG5-DBCOTris-GalNAc-β-Ala-PEG4-DBCONoOther:

Yield

Please select50 µg100 µg500 µg

QC

Purity by A260/A280 ratio and Integrity by denaturing Agarose Gel

Report

+ Add another Transcript

RNA Name

Template Source

DNA Length

RNA Length

Template Type

Please selectcircular plasmidlinearized plasmid (no digestion required)PCR product

To proceed with your request, it is required that your template contains a T7 promoter site. Please provide the sequence of the three bases immediately after the T7 promoter:

Full Sequence

Upload a file (Textfile (txt,Word), FASTA, GeneBank)

5’ Capping

Please selectNo capping Anti-Reverse CAP-Analogue (ARCA) Clean-Cap AG Other:

CleanCap AG requires the initiation sequence 5’ AGG 3’ ARCA Cap Analog requires the initiation sequence 5’ GG 3’

Phosphatase Treatment

Please selectYesNo

Uracil Modifications

Please selectUnmodified Pseudouridine N1-Methylpseudouridine Other: 

Poly(A) Tail

Please selectNo tail (RNA) Average length 20-150 Nt Included in the template 

Enzyme for Linearization

Buffer

Please select1 mM Sodium Citrate, pH 6.4 5 mM TRIS, pH 7.5 Other: 

3’ Modification via 3’-Azido-ddATP

Please selectYesNo

Click Conjugation at 3’end

Please selectDBCO-PEG4-5/6-FAMDBCO-PEG4-5/6-TAMRACy5-DBCOManα1-6(Manα1-3)Manα-Phenyl-PEG5-DBCOTris-GalNAc-β-Ala-PEG4-DBCONoOther:

Yield

Please select50 µg100 µg500 µg

QC

Purity by A260/A280 ratio and Integrity by denaturing Agarose Gel

Report

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