Intellectual Property

Link to the WO 2006/117161

This invention opens up new possibilities (methods, kits and reagents) for the simple and sensitive detection of samples, e.g. nucleic acids in complex biological samples using click chemistry.

Link to the WO 2008/052775

In order to increase the scope of the inventions from the previous patents, we have elaborated herein methods for the production of reporter molecules that can be used for analyte detection (e.g. nucleic acids in FISH experiments). A labeling method to introduce three different labels to the same oligonucleotides is disclosed.

Link to the WO 2010/115957

Click reactions employing CuBr as a catalyst suffer often from instability of this Cu(I) source, which renders fresh CuBr aliquots for each reaction setup necessary. We have a simple solution: a long-term air-stable heterogeneous Cu(I) source for easy click reactions and a device to perform the reactions in.

Link to the WO 2014/009429

Targeted delivery of therapeutic oligonucleotides is a key challenge for this new class of drugs. Here we disclose that anandamide-modified oligonucleotides are efficiently produced via click chemistry and can be taken up by neuronal and mammalian cells for siRNA mediated gene-knockdown.

Link to the 2015/107115

(Targeted) delivery of oligonucleotides for transient gene therapies is a major challenge. Here transfection agent free delivery of saccharide-modified oligonucleotides into plant (and mammalian cells) is disclosed. The oligonucleotide sugar conjugates are produced by click chemistry and could help to screen libraries of conjugates for a medicinal chemistry approach to RNA therapeutics.

Link to the WO 2019/063803

Enzymatic ligation is a key process in sample preparation for cloning and next-generation sequencing. The current ligases cannot efficiently ligate two single-stranded DNA oligonucleotides, thus limiting ligation of nucleic acid molecules mostly to double-stranded DNA. This invention discloses convenient procedures to integrate a chemical ligation protocol into current NGS library preparation workflows.

Link to the WO 2019/121803 A1

mRNAs are an emerging class of drugs with great promise, which face several challenges for commercialization. This is mostly due to a very limited capability for chemical modification during in vitro transcription of mRNA. Therefore, we developed a modular mRNA labeling method that can generate (multi-) modified functional mRNA molecules with improved properties.

Link to the WO 2021/130151

Whole transcriptome or exome sequencing has contributed enormously to understanding common and rare diseases, but has remained a challenge nonetheless. For this reason, we have developed an inventive method specifically for making 1: 1 copies of a full length RNA library for sequencing and quantitative analysis. After reverse transcription of RNA, we use click chemistry to chemically ligate adapter primers to the cDNA sequences in high yield. This allows us to amplify unknown sequences from the 3 ‘end to the 5’ end without losing information.

Link to the WO 2021/186028