2-Ethynyl-ATP (2-EATP) is a modified nucleotide that is commonly used in enzymatic assays intended to analyse the binding of ATP to proteins or it can be used to investigate ATP-depended enzymes in various pathological conditions. 2-EATP is also suitable for in vitro polyadenylation of natural RNA by recombinant poly(A) polymerase. The click reaction can be performed under the same conditions as before.

LITERATURE

Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures, F. Chen et al., 2019, Nucleic Acids Research, Vol. 47(22), p. e145.

https://doi.org/10.1093/nar/gkz852

Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap, D. Curanovic et al., 2013, Nature Chemical Biology, Vol. 9, p. 671–673.

https://doi.org/10.1038/nchembio.1334

A Platform for Controlled Template-Independent Enzymatic Synthesis of RNA Oligonucleotides and Therapeutics, D. J. Wiegand et al., 2023, bioRxiv, 2023-06.

https://doi.org/10.1101/2023.06.29.547106