Triphosphate for modifying of 3'END RNA or ssDNA
- Catalog No.
- 1 µmol
- € 250,00
- 5 µmol
- € 850,00
Azide modified nucleotide closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the azide group, 3′-Azido-2′,3′-ddTTP is well accepted by T7 RNA polymerase and poly(A) polymerase and terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available. Internal and external studies successfully showed e.g. that the poly(A) polymerase adds specifically and sequence independently single azido terminator to the 3’-end of RNA.
The main advantage: Any mRNA can be site-specifically labeled without special requirements or altered production protocols at the 3’-end.
Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry, M. L. Winz et al., 2012, Nucleic Acids Res., Vol. 40, p. 1–13.
Chemoenzymatic Preparation of Functional Click-Labeled Messenger RNA, S. Croce et al., 2020, ChemBioChem, Vol. 21, p. 1641-1646.
Application and design considerations for 3′-end sequencing using click-chemistry, M. K. Jensen et al., 2021, Methods in Enzymology, Vol. 655, p. 1-23.
12 months unopened after receipt
≥ 95% (HPLC)
100 mM clear aquaeous solution; colorless
149022-21-9 (sodium salt)
92586-35-1 (free acid)
λmax = 267 nm
3′-Azido-ddTTP, AZTTP, AZT-TP, Zidovudine
Triphosphate, Azidothymidine Triphosphate
- Molecular Formula