We enable nucleic acid labeling bioconjugation
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5-Ethynyl-dU-CEP

Clickable Oligo Synthesis Building Block

Currency:  
  • Size
  • Catalog No.
  • Price
  • 100 µmol (75.48 mg)
  • BCA-09-100

  • € 250,00
  • 250 mg
  • BCA-09-250

  • € 580,00
  • 1.0 g
  • BCA-09-1g

  • € 1.800,00
Clear
  • 5-Ethynyl-dU-CEP is a universal alkyne modifier that can be used under standard conditions for solid phase synthesis of oligonucleotides. It has shown to be incorporated into DNA oligomers with high coupling efficiency. Anyhow, the synthesis of an EdU oligo with a 5-Ethynyl-dU-CEP leads to some extent to a side product that can´t be purified with a normal HPLC. If you need the oligo in a higher purity, we suggest using TIPS -protected EdU-CEP (BCA-25). The oligo will be deprotected after the synthesis leading to the same product without the side product.

    LITERATURE

    D. Graham, J. a. Parkinson, T. Brown, J. Chem. Soc. Trans. 1 1998, 1131–1138.

    N. Minakawa, Y. Ono, A. Matsuda, J. Am. Chem. Soc. 2003, 125, 11545–11552.

    S. A. Ingale, H. Mei, P. Leonard, F. Seela, J. Org. Chem. 2013, 78, 11271–11282.

    F. Tolle, M. Rosenthal, F. Pfeiffer, G. Mayer, Bioconjug. Chem. 2016, 27, 500–503.

    • Molecular Formula

      C41H47N4O8P

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20°C dry, inert gas

    • Molecular Weight

      754.81 g/mol

    • Purity

      ≥ 90% (HPLC & 31P-NMR)

    • Physical State

      pale brown colored fluffy solid

    • CAS Number

      615288-66-9

    • Additional name

      5-ETHYNYL-DU CEP; 2′-Deoxy-5′-DMT-5-ethynyluridine 3′-CE phosphoramidite; 5′-O-[Bis(4-methoxyphenyl)(phenyl)methyl]-3′-O-[(2-cyanoethoxy)(diisopropylamino)phosphino]-2′-deoxy-5-ethynyluridine; 5′-O-[Bis(4-methoxyphenyl)phenylmethyl]-2′-deoxy-5-ethynyl-uridine 3′-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]

    • Preparation/Handling

      This phosphoramidite is used under standard conditions for solid phase synthesis of oligonucleotides.

    • Solubility

      MeCN

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