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5-Ethynyl-dU-CEP
Clickable Oligo Synthesis Building Block
- Size
- Catalog No.
- Price
- 100 µmol (75.48 mg)
BCA-09-100
- € 250,00
- 250 mg
BCA-09-250
- € 580,00
- 1.0 g
BCA-09-1g
- € 1.800,00
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5-Ethynyl-dU-CEP is a universal alkyne modifier that can be used under standard conditions for solid phase synthesis of oligonucleotides. It has shown to be incorporated into DNA oligomers with high coupling efficiency. Anyhow, the synthesis of an EdU oligo with a 5-Ethynyl-dU-CEP leads to some extent to a side product that can´t be purified with a normal HPLC. If you need the oligo in a higher purity, we suggest using TIPS -protected EdU-CEP (BCA-25). The oligo will be deprotected after the synthesis leading to the same product without the side product.
LITERATURE
D. Graham, J. a. Parkinson, T. Brown, J. Chem. Soc. Trans. 1 1998, 1131–1138.
N. Minakawa, Y. Ono, A. Matsuda, J. Am. Chem. Soc. 2003, 125, 11545–11552.
S. A. Ingale, H. Mei, P. Leonard, F. Seela, J. Org. Chem. 2013, 78, 11271–11282.
F. Tolle, M. Rosenthal, F. Pfeiffer, G. Mayer, Bioconjug. Chem. 2016, 27, 500–503.
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Molecular Formula
C41H47N4O8P
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C dry, inert gas
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Molecular Weight
754.81 g/mol
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Purity
≥ 90% (HPLC & 31P-NMR)
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Physical State
white to light brown solid
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CAS Number
615288-66-9
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Additional name
5-ETHYNYL-DU CEP; 2′-Deoxy-5′-DMT-5-ethynyluridine 3′-CE phosphoramidite; 5′-O-[Bis(4-methoxyphenyl)(phenyl)methyl]-3′-O-[(2-cyanoethoxy)(diisopropylamino)phosphino]-2′-deoxy-5-ethynyluridine; 5′-O-[Bis(4-methoxyphenyl)phenylmethyl]-2′-deoxy-5-ethynyl-uridine 3′-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]
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Preparation/Handling
This phosphoramidite is used under standard conditions for solid phase synthesis of oligonucleotides.
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Solubility
MeCN
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Molecular Formula