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5-Ethynyl-dUTP
Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Size
- Catalog No.
- Price
- 1.0 µmol
BCT-08-S
- € 100,00
- 5.0 µmol
BCT-08-L
- € 320,00
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5′-Ethynyl-dNTP can be easily incorporated into DNA by PCR using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified (we recommend a modification of 1-10% of the corresponding natural base only) PCR fragment is now ready for further functionalization with a.g. dyes, biomolecules, oligos, etc.
LITERATURE
J. Gierlich, K. Gutsmiedl, P. M. E. Gramlich, A. Schmidt, G. A. Burley, T. Carell, Chem. – A Eur. J. 2007, 13, 9486–9494.
G. A. Burley, J. Gierlich, M. R. Mofid, H. Nir, S. Tal, Y. Eichen, T. Carell, J. Am. Chem. Soc. 2006, 128, 1398–1399.
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Molecular Formula
C11H15N2O14P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
492.16 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
100 mM clear colorless solution in water (pH 7.5)
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CAS Number
111289-87-3
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Additional name
EdUTP; 5-Ethynyl-2’-deoxyuridine-5’-triphosphate
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Absorption (max)
λmax = 288 nm
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Ɛ (max)
12,000 cm-1M-1
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Molecular Formula