We enable nucleic acid labeling bioconjugation
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5-Ethynyl-UTP (5-EUTP)

Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1 µmol
  • BCT-07-S

  • € 250,00
  • 5 µmol
  • BCT-07-L

  • € 1.100,00
Clear
  • 5-Ethynyl-UTP (5-EUTP) can be incorporated into RNA using standard nucleotide mixtures and T7 RNA polymerase in vitro transcription. For a partial modified RNA we recommend to replace the natural base by only 1-10%. Modified mRNA is ready for labeling with marker azides (e.g. fluorescent dyes for tracking and imaging or cross-linking to other biomolecules) by click chemistry.

     

    LITERATURE

    Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation, S. Presolski et al., 2011, Current Protocols of Chemical Biology, Vol. 3, p. 153-162.

    https://doi.org/10.1002/9780470559277.ch110148

    Chemoenzymatic Preparation of Functional Click-Labeled Messenger RNA, S. Croce et al., 2020, ChemBioChem, Vol. 21, p. 1641-1646.

    https://doi.org/10.1002/cbic.201900718

    ALS-linked FUS mutants affect the localization of U7 snRNP and replication-dependent histone gene expression in human cells, A. Gadgil et al., 2021, Scientific Reports, Vol. 11(1), p. 11868.

    https://doi.org/10.1038/s41598-021-91453-3

    • Molecular Formula

      C11H15N2O15P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      507.97 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      100 mM clear aquaeous solution; colorless to slightly yellow

    • CAS Number

      1193451-16-9 (sodium salt)

      1004955-96-7 (free acid)

    • Absorption (max)

      λmax = 288 nm

    • Ɛ (max)

      12,000 cm-1M-1

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