Product Application for...
Product Emission color
Product Enatiomeric structure
Product Kits for..
Product Label Position
Product Linker type
Product Modification Type
Product Nucleoside
Product Nucleosites
Product Protection Type
Product Sort by
Product Sugar core
5-Ethynyl-UTP (5-EUTP)
Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Size
- Catalog No.
- Price
- 1 µmol
BCT-07-S
- € 250,00
- 5 µmol
BCT-07-L
- € 1.100,00
-
5-Ethynyl-UTP (5-EUTP) can be incorporated into RNA using standard nucleotide mixtures and T7 RNA polymerase in vitro transcription. For a partial modified RNA we recommend to replace the natural base by only 1-10%. Modified mRNA is ready for labeling with marker azides (e.g. fluorescent dyes for tracking and imaging or cross-linking to other biomolecules) by click chemistry.
LITERATURE
Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation, S. Presolski et al., 2011, Current Protocols of Chemical Biology, Vol. 3, p. 153-162.
https://doi.org/10.1002/9780470559277.ch110148
Chemoenzymatic Preparation of Functional Click-Labeled Messenger RNA, S. Croce et al., 2020, ChemBioChem, Vol. 21, p. 1641-1646.
https://doi.org/10.1002/cbic.201900718
ALS-linked FUS mutants affect the localization of U7 snRNP and replication-dependent histone gene expression in human cells, A. Gadgil et al., 2021, Scientific Reports, Vol. 11(1), p. 11868.
-
-
Molecular Formula
C11H15N2O15P3
-
Shelf Life
12 months unopened after receipt
-
Storage Conditions
-20 °C
-
Molecular Weight
507.97 g/mol
-
Purity
≥ 95% (HPLC)
-
Physical State
100 mM clear aquaeous solution; colorless to slightly yellow
-
CAS Number
1193451-16-9 (sodium salt)
1004955-96-7 (free acid)
-
Absorption (max)
λmax = 288 nm
-
Ɛ (max)
12,000 cm-1M-1
-
Molecular Formula