5-Ethynyl-UTP (5-EUTP)
Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
| Size | Catalog No. | Price |
|---|---|---|
| 1 µmol | BCT-07-S | € 320,00 |
| 5 µmol | BCT-07-L | € 1.200,00 |
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5-Ethynyl-UTP (5-EUTP) can be incorporated into RNA using standard nucleotide mixtures and T7 RNA polymerase in vitro transcription. For a partial modified RNA we recommend to replace the natural base by only 1-10%. Modified mRNA is ready for labeling with marker azides (e.g. fluorescent dyes for tracking and imaging or cross-linking to other biomolecules) by click chemistry.
LITERATURE
Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation, S. Presolski et al., 2011, Current Protocols of Chemical Biology, Vol. 3, p. 153-162.
https://doi.org/10.1002/9780470559277.ch110148
Chemoenzymatic Preparation of Functional Click-Labeled Messenger RNA, S. Croce et al., 2020, ChemBioChem, Vol. 21, p. 1641-1646.
https://doi.org/10.1002/cbic.201900718
ALS-linked FUS mutants affect the localization of U7 snRNP and replication-dependent histone gene expression in human cells, A. Gadgil et al., 2021, Scientific Reports, Vol. 11(1), p. 11868.
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Molecular Formula
C11H15N2O15P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
507.97 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
100 mM clear aquaeous solution; colorless to slightly yellow
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CAS Number
1193451-16-9 (sodium salt)
1004955-96-7 (free acid)
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Absorption (max)
λmax = 288 nm
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Ɛ (max)
12,000 cm-1M-1
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Molecular Formula