Modified triphosphate for incorporation in PCR reaction
- Catalog No.
- 1 µmol
- € 85,00
- 5 µmol
- € 260,00
C8-Alkyne-dUTP is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dUTP.
Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. – A Eur. J., Vol. 13, p. 9486–9494.
Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128, p. 1398–1399.
Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction, S. Hesse et al., 2016, Chromosome Research, Vol. 24(3), p. 299–307.
Enzymatic Synthesis of Chemical Nuclease Triplex-Forming Oligonucleotides with Gene-Silencing Applications, B. McGorman et al., 2022, Nucleic Acids Research, Vol. 50(10), p. 5467–5481.
12 months unopened after receipt
≥ 95% (HPLC)
100 mM clear colorless
solution in water (pH 7.5)
1004297-65-7 (free acid)
λmax = 292 nm
- Molecular Formula