We enable nucleic acid labeling bioconjugation
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C8-Alkyne-dUTP

Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides

Currency:  
  • Size
  • Catalog No.
  • Price
  • 0.5 µmol
  • BCT-05-S

  • € 75,00
  • 2.5 µmol
  • BCT-05-M

  • € 120,00
  • 5.0 µmol
  • BCT-05-L

  • € 240,00
Clear
  • This modified nucleotide triphosphate can be used in standard nucleotide mixtures to label your PCR-fragments with our marker azides. The incorporation of clickable C8-Alkyne dNTPs is accepted by broad polymerase (e.g. with Pwo, Deep Vent exo- or KOD XL) in PCR. As the alkyne introduction as well as the following labeling step by click chemistry can be so effective, we recommend to not replace the natural base by 100% with the modified one in order not to alter the properties of your PCR fragment too much.

    LITERATURE

    J. Gierlich, K. Gutsmiedl, P. M. E. Gramlich, A. Schmidt, G. A. Burley, T. Carell, Chem. – A Eur. J. 2007, 13, 9486–9494.

    G. A. Burley, J. Gierlich, M. R. Mofid, H. Nir, S. Tal, Y. Eichen, T. Carell, J. Am. Chem. Soc. 2006, 128, 1398–1399.

    • Molecular Formula

      C17H23N2O14P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      572.29 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      100 mM clear colorless
      solution in water (pH 7.5)

    • CAS Number

      n.a.

    • Absorption (max)

      λmax = 292 nm

    • Ɛ (max)

      11.000 cm-1M-1

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