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C8-Alkyne-dUTP

Modified triphosphate for incorporation in PCR reaction

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  • Size
  • Catalog No.
  • Price
  • 1 µmol
  • BCT-05-S

  • € 100,00
  • 5 µmol
  • BCT-05-L

  • € 300,00
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  • C8-Alkyne-dUTP is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dUTP.

     

    LITERATURE

    Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. – A Eur. J., Vol. 13, p. 9486–9494.

    https://doi.org/10.1002/chem.200700502

    Directed DNA Metallization, G. A. Burley et al., 2006J. Am. Chem. Soc., Vol. 128, p. 1398–1399.

    https://doi.org/10.1021/ja055517v

    Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction, S. Hesse et al., 2016, Chromosome Research, Vol. 24(3), p. 299–307.

    https://doi.org/10.1007/s10577-016-9522-z

    Enzymatic Synthesis of Chemical Nuclease Triplex-Forming Oligonucleotides with Gene-Silencing Applications, B. McGorman et al., 2022, Nucleic Acids Research, Vol. 50(10), p. 5467–5481.

    https://doi.org/10.1093/nar/gkac438

    • Molecular Formula

      C17H23N2O14P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      572.29 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      100 mM clear colorless
      solution in water (pH 7.5)

    • CAS Number

      1004297-65-7 (free acid)

    • Absorption (max)

      λmax = 292 nm

    • Ɛ (max)

      11.000 cm-1M-1

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