C8-Alkyne-dUTP is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dUTP.

LITERATURE

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https://doi.org/10.1002/chem.200700502

Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128, p. 1398–1399.

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Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction, S. Hesse et al., 2016, Chromosome Research, Vol. 24(3), p. 299–307.

https://doi.org/10.1007/s10577-016-9522-z

Enzymatic Synthesis of Chemical Nuclease Triplex-Forming Oligonucleotides with Gene-Silencing Applications, B. McGorman et al., 2022, Nucleic Acids Research, Vol. 50(10), p. 5467–5481.

https://doi.org/10.1093/nar/gkac438