ClickTech Library Kit full-length mRNA_Seq V2.0

baseclick’s ClickTech Library Kit full-length mRNA_Seq V2.0 has been developed as a method for sequencing total mRNA or the whole exome of cells for genetic diagnosis. The kit provides reagents for cDNA synthesis from any RNA pool and introduces a single azide nucleotide at the 3’End of cDNA. This azido-modified cDNA can react with an 5’-alkyne-modified adapter sequence in a highly specific and biorthogonal manner under benign click reaction conditions. With the included specially designed PCR Primers, the 3’-adapter-clicked cDNA can be amplified via PCR, enabling subsequent long-read Third Generation Sequencing experiments or alternatively targeted gene amplifications