baseclick’s ClickTech Library Kit full-length mRNA_Seq V2.0 has been developed as a method for sequencing total mRNA of cells for transcriptome analysis. The kit provides reagents for cDNA synthesis from any RNA pool and introduces a single azide nucleotide at the 3’End of cDNA. This azido-modified cDNA can react with an 5’-alkyne-modified adapter sequence in a highly specific and biorthogonal manner under benign click reaction conditions. With the included specially designed PCR Primers, the 3’-adapter-clicked cDNA can be amplified via PCR, enabling subsequent NGS experiments or alternatively targeted gene amplifications

LITERATURE

Click Chemistry Enables Rapid Amplification of Full-Length Reverse Transcripts for Long-Read Third Generation Sequencing, E. S. Schönegger et al., 2022, Bioconjugate Chem., Vol. 33(10), p. 1789–1795.
https://pubs.acs.org/doi/10.1021/acs.bioconjchem.2c00353