ClickTech Oligo Link Kit

Click chemistry is an easy bioconjugation method with mild conditions and extreme high yields to link covalently biomolecules such RNA, DNA or LNA (etc.) to linker, tags, dyes, sugars, peptides etc. It is a quantitative chemical reactions of alkyne and azide moieties to create covalent bonds which is thermally and hydrolytically stable, ideal for oligonucleotide labeling. More information can be found in our learn section.

The ClickTech Oligo Link Kit includes solvents buffer and reactors optimized for the copper-catalyzed version of the Huisgen cycloaddition (CuAAC) between a terminal alkyne and an azide. It does not target strain-promoted acid-alkyne cycloaddition (SPAAC).

However, both types of oligo modifications and tags can be requested through our oligo calculator.

Oligo-Oligo ligation:

Solid phase synthesis is limited when it comes to the length of oligos. The price of long DNA or RNA oligos becomes very expensive above a certain base length or cannot be implemented because the yield of long oligos decreases exponentially with the length of the oligo.

Long oligos can be reached by enzymatic ligation using splint oligos, but resulting in bad yields and several purification steps are necessary. Our Click Tech Oligo Link Kit offers easy and high efficient chemical ligation and elongation order one oligo with a 3’modification and another with a 5’modification and ligate the two oligos together using click chemistry. baseclick will gladly provide you with suitably-modified oligos containing the necessary click functionality. Many modifications can be requested using oligo calculator.

Oligo-Tag Link:

Would you like to produce DNA FISH probes or molecular beacons for RT-qPCR with specific dyes? Are you interested in a DNA oligo to try out therapeutics and looking for oligos with targeting agents such as mannose or GalNAc? Then our ClickTech Oligo Link Kit is the right choice. We offer several linkers, tags and targeting agents to modify your oligonucleotide. If you need support with your project, please contact us.

Out of over 10 years of experience in the field of click technology and oligonucleotides we created a combination of buffers, ligands, agents and catalysts to meet your specific needs for oligonucleotide modifications. The kit provides the reagents needed to perform 10 reactions. For individual applications, the kit does not provide a modified oligonucleotide and no label azides or alkynes. All can be requested at baseclick, please contact us.

You can choose between two sizes, S and L. In the ClickTech Oligo Link Kit with the size S you can link oligonucleotides from 70 pmol to 22 nmol. If you wish to produce linked oligos from 1 nmol to 90 nmol then choose size L. These are the minimal and maximal amounts of reacted oligonucleotide that can be achieved using the kits.