ClickTech Oligo Link Kit
Kit for easy Labeling of Oligos for even Connecting / Ligating two Oligos
- Catalog No.
- Size S / 1 Kit
- € 55,00
- Size L / 1 Kit
- € 65,00
This ClickTech Oligo Link Kit is designed for two approaches. It includes reagents and a protocol to click-label your oligonucleotide with clickable tags or for oligonucleotide elongation with a further oligo. Enzymatic ligation is replaced by click ligation without further additions, such as typically splint oligonucleotides for pre-organization. Compared to the enzymatic alternative the “click ligation” exhibits several advantages: no byproducts, increased ligated product an additionally, purification of the desired product is straightforward. Furthermore, several enzymes as well as broad polymerase accepte the obtained triazole linkage in PCR.
What modifications does my oligos have to have in order to be clickable?
The oligos must have clickable modified building blocks that have either terminal alkynes or azido functional groups. A wide range of clickable amidites for solid phase synthesis can be found here. Or generate your desired oligo using our oligo calculator and purchase from us oligos like RNA, DNA or LNA in high quality. Select the desired azide or alkyne modification at the desired location within your nucleic acid sequence. We are happy to advise you if you want to order unusual oligos that we do not offer in our oligo calculator. Just contact us. There are also other oligo providers that have the license from us to produce such oligos. Feel free to contact us for information about different click-licensed providers.
What is click chemistry and what are its advantages over other methods in the field of labeling DNA or RNA?
Click chemistry is an easy bioconjugation method with mild conditions and extreme high yields to link covalently biomolecules such RNA, DNA or LNA (etc.) to linker, tags, dyes, sugars, peptides etc. It is a quantitative chemical reactions of alkyne and azide moieties to create covalent bonds which is thermally and hydrolytically stable, ideal for oligonucleotide labeling. More information can be found in our learn section.
Has the kit been designed for CuAAC and / or SPAAC?
The ClickTech Oligo Link Kit includes solvents buffer and reactors optimized for the copper-catalyzed version of the Huisgen cycloaddition (CuAAC) between a terminal alkyne and an azide. It does not target strain-promoted acid-alkyne cycloaddition (SPAAC).
However, both types of oligo modifications and tags can be requested through our oligo calculator.
What is the ClickTech Oligo Link Kit used for?
Solid phase synthesis is limited when it comes to the length of oligos. The price of long DNA or RNA oligos becomes very expensive above a certain base length or cannot be implemented because the yield of long oligos decreases exponentially with the length of the oligo.
Long oligos can be reached by enzymatic ligation using splint oligos, but resulting in bad yields and several purification steps are necessary. Our Click Tech Oligo Link Kit offers easy and high efficient chemical ligation and elongation order one oligo with a 3’modification and another with a 5’modification and ligate the two oligos together using click chemistry. baseclick will gladly provide you with suitably-modified oligos containing the necessary click functionality. Many modifications can be requested using oligo calculator.
Would you like to produce DNA FISH probes or molecular beacons for RT-qPCR with specific dyes? Are you interested in a DNA oligo to try out therapeutics and looking for oligos with targeting agents such as mannose or GalNAc? Then our ClickTech Oligo Link Kit is the right choice. We offer several linkers, tags and targeting agents to modify your oligonucleotide. If you need support with your project, please contact us.
What is in the ClickTech Oligo Link Kit?
Out of over 10 years of experience in the field of click technology and oligonucleotides we created a combination of buffers, ligands, agents and catalysts to meet your specific needs for oligonucleotide modifications. The kit provides the reagents needed to perform 10 reactions. For individual applications, the kit does not provide a modified oligonucleotide and no label azides or alkynes. All can be requested at baseclick, please contact us.
What is the difference between the ClickTech Oligo Link Kit S and L?
You can choose between two sizes, S and L. In the ClickTech Oligo Link Kit with the size S you can link oligonucleotides from 70 pmol to 22 nmol. If you wish to produce linked oligos from 1 nmol to 90 nmol then choose size L. These are the minimal and maximal amounts of reacted oligonucleotide that can be achieved using the kits.
- What modifications does my oligos have to have in order to be clickable?
12 months unopened after receipt
RT and – 20°C (kit contains two parts)
kit system made of different components
please see user manual of the kit
- Shelf Life