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Bifunctional linker

Size Catalog No. Price
5 mg BCL-014-5 € 40,00
10 mg BCL-014-10 € 65,00
  • NHS-C3-Azide is a hetero-bifunctional reagent that allows to crosslink alkyne (bio-)molecules to compounds bearing primary amines. The NHS ester group will react with primary amines while the azide can undergo click reaction. This linker is often used to introduce azide moieties into amino-oligonucleotides after solid phase synthesis.



    Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking, W. Hou et al., 2019Scientific Reports, Vol. 9, p. 1307.


    Integration of a field effect transistor-based aptasensor under a hydrophobic membrane for bioelectronic nose applications, A.E. Kuznetsov et al., 2019, Biosensors and Bioelectronics, Vol. 129, p. 29-35.


    Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity, P. Kumar et al., 2018J. Clin. Investig., Vol. 128(8), p. 3298-3311.


    Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase, M. Andrianova et al., 2018, Sensores, Vol. 18, p. 49.


    Aptamer based vanillin sensor using an ion-sensitive field-effect transistor, A. Kuznetsov et al., 2018, Microchimica Acta, Vol. 185(1), p. 3.


    Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence, M. Taskova et al., 2017, Analytical Chemistry, Vol. 89(8), p. 4363–4366.


    Gas phase click chemistry via ion/ion reactions, J. Bu et al., 2015, Int J Mass Spectrom, Vol. 309, p. 118-123.


    Two-step labeling of Staphylococcus aureus with Lysostaphin-Azide and DIBO-Alexa using click chemistry, I. Potapova et al., 2013, Journal of Microbiological Methods, Vol. 92(1), p. 90-98.


    • Molecular Formula


    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dry, inert gas

    • Molecular Weight

      226.19 g/mol

    • Purity

      ≥ 95% (NMR)

    • Physical State

      White to off-white solid

    • CAS Number


    • Additional name

      gamma-Azidobutyric acid oxysuccinimide ester; γ-Azidobutyric acid NHS; 2,5-dioxopyrrolidin-1-yl 4-azidobutanoate

    • Solubility

      DMSO, DMF, DCM

    • Preparation/Handling

      For a 10 mM solution add 442 μL to 1 mg.