We enable nucleic acid labeling bioconjugation
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NHS-C3-Azide

Bifunctional linker

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  • Size
  • Catalog No.
  • Price
  • 5 mg
  • BCL-014-5

  • € 40,00
  • 10 mg
  • BCL-014-10

  • € 65,00
Clear
  • NHS-C3-Azide is a hetero-bifunctional reagent that allows to crosslink alkyne (bio-)molecules to compounds bearing primary amines. The NHS ester group will react with primary amines while the azide can undergo click reaction. This linker is often used to introduce azide moieties into amino-oligonucleotides after solid phase synthesis.

     

    LITERATURE

    Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking, W. Hou et al., 2019Scientific Reports, Vol. 9, p. 1307.

    https://doi.org/10.1038/s41598-018-37789-9

    Integration of a field effect transistor-based aptasensor under a hydrophobic membrane for bioelectronic nose applications, A.E. Kuznetsov et al., 2019, Biosensors and Bioelectronics, Vol. 129, p. 29-35.

    https://doi.org/10.1016/j.bios.2019.01.013

    Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity, P. Kumar et al., 2018J. Clin. Investig., Vol. 128(8), p. 3298-3311.

    https://doi.org/10.1172/JCI97659DS1

    Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase, M. Andrianova et al., 2018, Sensores, Vol. 18, p. 49.

    https://doi.org/10.3390/s18010049

    Aptamer based vanillin sensor using an ion-sensitive field-effect transistor, A. Kuznetsov et al., 2018, Microchimica Acta, Vol. 185(1), p. 3.

    https://doi.org/10.1007/s00604-017-2586-4

    Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence, M. Taskova et al., 2017, Analytical Chemistry, Vol. 89(8), p. 4363–4366.

    https://doi.org/10.1021/acs.analchem.7b00646

    Gas phase click chemistry via ion/ion reactions, J. Bu et al., 2015, Int J Mass Spectrom, Vol. 309, p. 118-123.

    https://doi.org/10.1016/j.ijms.2015.05.010

    Two-step labeling of Staphylococcus aureus with Lysostaphin-Azide and DIBO-Alexa using click chemistry, I. Potapova et al., 2013, Journal of Microbiological Methods, Vol. 92(1), p. 90-98.

    https://doi.org/10.1016/j.mimet.2012.11.004

    • Molecular Formula

      C8H10N4O4

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dry, inert gas

    • Molecular Weight

      226.19 g/mol

    • Purity

      ≥ 95% (NMR)

    • Physical State

      White to off-white solid

    • CAS Number

      943858-70-6

    • Additional name

      gamma-Azidobutyric acid oxysuccinimide ester; γ-Azidobutyric acid NHS; 2,5-dioxopyrrolidin-1-yl 4-azidobutanoate

    • Solubility

      DMSO, DMF, DCM

    • Preparation/Handling

      For a 10 mM solution add 442 μL to 1 mg.

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