2′-Azido-2′-dATP
Triphosphate for modifying of 3'END RNA
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Azide modified nucleotide closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the azide group, 2′-Azido-2′-dATP is well accepted by T7 RNA polymerase and poly(A) polymerase and terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation at the 3’end. Modified mRNA is ready for labeling with alkyne- or DBCO containing reaction partners. The possibility of performing strain-promoted Azide-Alkyne chemistry (SPAAC) enables the copper free click labeling of RNA at the sugar. This is highly beneficial, as labeling of mRNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available.
LITERATURE
Bioorthogonal mRNA labeling at the poly (A) tail for imaging localization and dynamics in live zebrafish embryos, K. J. Westerich et al., 2020, Chemical Science, Vol. 11(11), p. 3089-3095.
https://doi.org/10.1039/C9SC05981D
Enzymatic Generation of Double‐Modified AdoMet Analogues and Their Application in Cascade Reactions with Different Methyltransferases, M. Erguven et al., 2022, ChemBioChem, Vol. 23(24), e202200511.
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Molecular Formula
C10H15N8O12P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark, dry
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Molecular Weight
532.19 g/mol (free acid)
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Purity
≥ 95% (HPLC)
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Physical State
colorless solution (100 mM, pH 7.5)
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CAS Number
69093-69-2
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Absorption (max)
λmax = 258 nm
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Ɛ (max)
15.400 cm-1M-1
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Molecular Formula