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3-Azido-7-hydroxycoumarin
Switch-on Dye to label DNA/RNA via click chemistry
- Size
- Catalog No.
- Price
- 1.0 mg
BCFA-047-1
- € 60,00
- 5.0 mg
BCFA-047-5
- € 150,00
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The 3-Azido-7-hydroxycoumarin has the special property to become fluorescent only when it is click ligated. The formed tiazole bond expands then the electron system of the dye and it lights up upon excitation. As basically background signal due to left over dye reagent is not visible, this dye is a valuable tool for nucleic acid labeling.
LITERATURE
Laughlin et al, Sci USA, 2009, 106 (1):12
Li et al, Biotechniques, 2010, 49 (1):525
Sivakumar et al, Org. Lett., 2004, 6 (24):4603
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Molecular Formula
C9H5N3O3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
203.15 g/mol
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Purity
≥ 98% (HPLC)
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Physical State
amber to brown crystalline solid
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CAS Number
817638-68-9
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Additional name
3-Azido-7-hydroxy-chromen-2-on; 3-Azido-7-
hydroxy-chroman-2-one -
Excitation (max)
before click reaction: 260 nm
after click reaction: 404 nm -
Emission (max)
before click reaction: 391 nm
after click reaction: 480 nm -
Solubility
DMSO, DMF, MeOH, MeCN
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Preparation/Handling
For a 10 mM solution add 492 ÎĽL to 1mg.
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Molecular Formula