We enable nucleic acid labeling bioconjugation
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3-Azido-7-hydroxycoumarin

Switch-on Dye to label DNA/RNA via click chemistry

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1.0 mg
  • BCFA-047-1

  • € 90,00
  • 5.0 mg
  • BCFA-047-5

  • € 315,00
Clear
  • The 3-Azido-7-hydroxycoumarin has the special property to become fluorescent only when it is click ligated. The formed tiazole bond expands then the electron system of the dye and it lights up upon excitation. As basically background signal due to left over dye reagent is not visible, this dye is a valuable tool for nucleic acid labeling.

    LITERATURE

    Laughlin et al, Sci USA, 2009, 106 (1):12

    Li et al, Biotechniques, 2010, 49 (1):525

    Sivakumar et al, Org. Lett., 2004, 6 (24):4603

    • Molecular Formula

      C9H5N3O3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      203.15 g/mol

    • Purity

      ≥ 98% (HPLC)

    • Physical State

      amber to brown crystalline solid

    • CAS Number

      817638-68-9

    • Additional name

      3-Azido-7-hydroxy-chromen-2-on; 3-Azido-7-
      hydroxy-chroman-2-one

    • Absorption (max)

      before click reaction: 260 nm
      after click reaction: 404 nm

    • Emission (max)

      before click reaction: 391 nm
      after click reaction: 480 nm

    • Solubility

      DMSO, DMF, MeOH, MeCN

    • Preparation/Handling

      For a 10 mM solution add 492 μL to 1mg.