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Switch-on dye to label DNA/RNA

Size Catalog No. Price
1 mg BCFA-047-1  50,00
5 mg BCFA-047-5  135,00
  • 3-Azido-7-hydroxycoumarin has the special property to become fluorescent only when it is click ligated. The formed triazole expands the electron system of the dye and enables fluorescence. Therefore, there is no background signal of the click unligated leftover dye. This dye is a valuable tool for nucleic acid labeling.



    Imaging the glycome, Laughlin et al, 2009Sci USA, Vol. 106, p. (1):12


    Fluorogenic “click” reaction for labeling and detection of DNA in proliferating cells, Li et al, 2010Biotechniques, Vol. 49, p. (1):525


    A Fluorogenic 1,3-Dipolar Cycloaddition Reaction of 3-Azidocoumarins and Acetylenes, Sivakumar et al, 2004Org. Lett., Vol. 6, p. (24):4603


    Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry, C. Siverino et al., 2018, Journal of Visualized Experiments, Vol. 133, e56616.


    Characterization and Scaled-Up Production of Azido-Functionalized Silk Fiber Produced by Transgenic Silkworms with an Expanded Genetic Code, H. Teramoto et al., 2019, International Journal of Molecular Sciences, Vol. 20(3), p. 616.


    A lipid transfer protein knockout library reveals ORP9-ORP11 dimer mediating PS/PI(4)P exchange at the ER-trans Golgi contact site to promote sphingomyelin synthesis, B. Cabukusta et al., 2023, bioRxiv.


    Trifunctional lipid probes for comprehensive studies of single lipid species in living cells, D. Höglinger, 2017, Proceedings of the National Academy of Sciences, Vol. 114(7), p. 1566-1571.


    • Molecular Formula


    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      203.15 g/mol

    • Purity

      ≥ 98% (HPLC)

    • Physical State

      amber to brown crystalline solid

    • CAS Number


    • Additional name

      3-Azido-7-hydroxy-chromen-2-on; 3-Azido-7-

    • Excitation (max)

      before click reaction: 260 nm
      after click reaction: 404 nm

    • Emission (max)

      before click reaction: 391 nm
      after click reaction: 480 nm

    • Solubility

      DMSO, DMF, MeOH, MeCN

    • Preparation/Handling

      For a 10 mM solution add 492 μL to 1 mg.