3-Azido-7-hydroxycoumarin

3-Azido-7-hydroxycoumarin has the special property to become fluorescent only when it is click ligated. The formed triazole expands the electron system of the dye and enables fluorescence. Therefore, there is no background signal of the click unligated leftover dye. This dye is a valuable tool for nucleic acid labeling.

LITERATURE

Imaging the glycome, Laughlin et al, 2009, Sci USA, Vol. 106, p. (1):12

https://doi.org/10.1073/pnas.0811481106

Fluorogenic “click” reaction for labeling and detection of DNA in proliferating cells, Li et al, 2010, Biotechniques, Vol. 49, p. (1):525

https://doi.org/10.2144/000113463

A Fluorogenic 1,3-Dipolar Cycloaddition Reaction of 3-Azidocoumarins and Acetylenes, Sivakumar et al, 2004, Org. Lett., Vol. 6, p. (24):4603

https://doi.org/10.1021/ol047955x

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry, C. Siverino et al., 2018, Journal of Visualized Experiments, Vol. 133, e56616.

https://doi.org/10.3791/56616

Characterization and Scaled-Up Production of Azido-Functionalized Silk Fiber Produced by Transgenic Silkworms with an Expanded Genetic Code, H. Teramoto et al., 2019, International Journal of Molecular Sciences, Vol. 20(3), p. 616.

https://doi.org/10.3390/ijms20030616

A lipid transfer protein knockout library reveals ORP9-ORP11 dimer mediating PS/PI(4)P exchange at the ER-trans Golgi contact site to promote sphingomyelin synthesis, B. Cabukusta et al., 2023, bioRxiv.

https://doi.org/10.1101/2023.06.02.543249

Trifunctional lipid probes for comprehensive studies of single lipid species in living cells, D. Höglinger, 2017, Proceedings of the National Academy of Sciences, Vol. 114(7), p. 1566-1571.

https://doi.org/10.1073/pnas.1611096114