We enable nucleic acid labeling bioconjugation
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5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

TAMRA fluorescent dye to label DNA/RNA via click chemistry

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  • Size
  • Catalog No.
  • Price
  • 5 mg
  • BCFA-008-5

  • € 100,00
  • 10 mg
  • BCFA-008-10

  • € 150,00
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  • 5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide) is a reporter reagent that allows efficient fluorescent labeling of nucleic acids by click bioconjugation. It is often used as FRET-acceptor in combination with FAM. Just like many rhodamine-based dyes, it is quite photostable between pH 4 and 9. It shows identical spectral properties to Alexa Fluor® 555 or DyLight 549 dyes.

     

    LITERATURE

    Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.

    https://doi.org/10.1002/anie.200802077

    Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.

    https://doi.org/10.1002/anie.200705664

    A chemical method for fast and sensitive detection of DNA synthesis in vivo, A. Salic et al., 2008, Proc. Natl. Acad. Sci. U. S. A., Vol. 105, p. 2415–2420.

    https://doi.org/10.1073/pnas.0712168105

    Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction, S. Hesse et al., 2016, Chromosome Research, Vol. 24, p. 299–307.

    https://doi.org/10.1007/s10577-016-9522-z

    Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation, A. Borrmann et al., 2012, ChemBioChem, Vol. 13(14), p. 2094-2099.

    https://doi.org/10.1002/cbic.201200407

    New insights into the intracellular distribution pattern of cationic amphiphilic drugs, M. Vater et al., 2017, Scientific Reports, Vol 7, p. 44277.

    https://doi.org/10.1038/srep44277

    The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain, I. V. L. Wilkinson et al., 2022, ChemBioChem, Vol. 24(5), p. e202200455.

    https://doi.org/10.1002/cbic.202200455

    • Molecular Formula

      C28H28N6O4

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      512.56 g/mol

    • Purity

      ≥ 95% (LCMS)

    • Physical State

      pink to dark red colored solid

    • CAS Number

      1192590-89-8

    • Additional name

      5-TAMRA-Azide; 5-Carboxytetramethylrhodamine Azide, 5-((3-Azidopropyl)carbamoyl)-2-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)benzoate

    • Excitation (max)

      DMSO or DMF: 546 nm

    • Emission (max)

      DMSO or DMF: 579 nm

    • Ɛ (max)

      DMSO or DMF = 91,000 cm-1M-1

    • Solubility

      DMSO, DMF, MeOH

    • Preparation/Handling

      For a 10 mM solution add 195 μL to 1 mg.

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