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5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)
TAMRA Fluorescent dye to label DNA/RNA
- Size
- Catalog No.
- Price
- 5.0 mg
BCFA-008-5
- € 90,00
- 10 mg
BCFA-008-10
- € 135,00
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5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide) is a marker reagent that allows efficient fluorescent labeling of nucleic acids by click bioconjugation. It is often used as FRET-acceptor in combination with FAM. Just like many rhodamine-based dyes, it is quite photostable between pH 4 and 9. It shows identical to almost identical spectral properties to Alexa Fluor® 555 or DyLight 549 dyes.
LITERATURE
P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chemie – Int. Ed. 2008, 47, 8350–8358.
P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, 2008.
A. Salic, T. J. Mitchison, Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 2415–2420.
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Molecular Formula
C28H28N6O4
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
512.56 g/mol
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Purity
≥ 95% (LCMS)
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Physical State
pink to dark red colored powder
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CAS Number
1192590-89-8
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Additional name
5-TAMRA-Azide; 5-Carboxytetramethylrhodamine Azide, 5-((3-Azidopropyl)carbamoyl)-2-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)benzoate
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Excitation (max)
DMSO or DMF: 546 nm
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Emission (max)
DMSO or DMF: 579 nm
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Ɛ (max)
DMSO or DMF = 91,000 cm-1M-1
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Solubility
DMSO, DMF, MeOH
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Preparation/Handling
For a 10 mM solution add 195 μL to 1 mg.
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Molecular Formula