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5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

TAMRA fluorescent dye to label DNA/RNA

Size Catalog No. Price
5 mg BCFA-008-5  100,00
10 mg BCFA-008-10  150,00
  • 5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide) is a reporter reagent that allows efficient fluorescent labeling of nucleic acids by click bioconjugation. It is often used as FRET-acceptor in combination with FAM. Just like many rhodamine-based dyes, it is quite photostable between pH 4 and 9. It shows identical spectral properties to Alexa Fluor® 555 or DyLight 549 dyes.



    Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.


    Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.


    A chemical method for fast and sensitive detection of DNA synthesis in vivo, A. Salic et al., 2008, Proc. Natl. Acad. Sci. U. S. A., Vol. 105, p. 2415–2420.


    Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction, S. Hesse et al., 2016, Chromosome Research, Vol. 24, p. 299–307.


    Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation, A. Borrmann et al., 2012, ChemBioChem, Vol. 13(14), p. 2094-2099.


    New insights into the intracellular distribution pattern of cationic amphiphilic drugs, M. Vater et al., 2017, Scientific Reports, Vol 7, p. 44277.


    The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain, I. V. L. Wilkinson et al., 2022, ChemBioChem, Vol. 24(5), p. e202200455.


    • Molecular Formula


    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      512.56 g/mol

    • Purity

      ≥ 95% (LCMS)

    • Physical State

      pink to dark red colored solid

    • CAS Number


    • Additional name

      5-TAMRA-Azide; 5-Carboxytetramethylrhodamine Azide, 5-((3-Azidopropyl)carbamoyl)-2-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)benzoate

    • Excitation (max)

      DMSO or DMF: 546 nm

    • Emission (max)

      DMSO or DMF: 579 nm

    • Ɛ (max)

      DMSO or DMF = 91,000 cm-1M-1

    • Solubility

      DMSO, DMF, MeOH

    • Preparation/Handling

      For a 10 mM solution add 195 μL to 1 mg.