5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide) is a reporter reagent that allows efficient fluorescent labeling of nucleic acids by click bioconjugation. It is often used as FRET-acceptor in combination with FAM. Just like many rhodamine-based dyes, it is quite photostable between pH 4 and 9. It shows identical spectral properties to Alexa Fluor® 555 or DyLight 549 dyes.

LITERATURE

Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.

https://doi.org/10.1002/anie.200802077

Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.

https://doi.org/10.1002/anie.200705664

A chemical method for fast and sensitive detection of DNA synthesis in vivo, A. Salic et al., 2008, Proc. Natl. Acad. Sci. U. S. A., Vol. 105, p. 2415–2420.

https://doi.org/10.1073/pnas.0712168105

Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction, S. Hesse et al., 2016, Chromosome Research, Vol. 24, p. 299–307.

https://doi.org/10.1007/s10577-016-9522-z

Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation, A. Borrmann et al., 2012, ChemBioChem, Vol. 13(14), p. 2094-2099.

https://doi.org/10.1002/cbic.201200407

New insights into the intracellular distribution pattern of cationic amphiphilic drugs, M. Vater et al., 2017, Scientific Reports, Vol 7, p. 44277.

https://doi.org/10.1038/srep44277

The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain, I. V. L. Wilkinson et al., 2022, ChemBioChem, Vol. 24(5), p. e202200455.

https://doi.org/10.1002/cbic.202200455