We enable nucleic acid labeling bioconjugation
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5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

TAMRA Fluorescent dye to label DNA/RNA

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1.0 mg
  • BCFA-008-1

  • € 60,00
  • 5.0 mg
  • BCFA-008-5

  • € 80,00
  • 10 mg
  • BCFA-008-10

  • € 125,00
  • 50 mg
  • BCFA-008-50

  • € 320,00
  • 100 mg
  • BCFA-008-100

  • € 500,00
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  • 5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide) is a marker reagent that allows efficient fluorescent labeling of nucleic acids by click bioconjugation. It is often used as FRET-acceptor in combination with FAM. Just like many rhodamine-based dyes, it is quite photostable between pH 4 and 9. It shows identical to almost identical spectral properties to Alexa Fluor® 555 or DyLight 549 dyes.

    LITERATURE

    P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chemie – Int. Ed. 2008, 47, 8350–8358.

    P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, 2008.

    A. Salic, T. J. Mitchison, Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 2415–2420.

    • Molecular Formula

      C28H28N6O4

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      512.56 g/mol

    • Purity

      ≥ 95% (LCMS)

    • Physical State

      pink to dark red colored powder

    • CAS Number

      1192590-89-8

    • Additional name

      5-TAMRA-Azide; 5-Carboxytetramethylrhodamine Azide, 5-((3-Azidopropyl)carbamoyl)-2-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)benzoate

    • Absorption (max)

      DMSO or DMF: 546 nm

    • Emission (max)

      DMSO or DMF: 579 nm

    • Ɛ (max)

      DMSO or DMF = 91,000 cm-1M-1

    • Solubility

      DMSO, DMF, MeOH

    • Preparation/Handling

      For a 10 mM solution add 195 μL to 1 mg.

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