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5-Ethynyl-2′-deoxyuridine (5-EdU)

Clickable EdU allows detection of de novo DNA

Size Catalog No. Price
5 mg BCN-001-5  20,00
50 mg BCN-001-50  65,00
100 mg BCN-001-100  100,00
500 mg BCN-001-500  400,00
1 g BCN-001-1g  680,00
  • 5-Ethynyl-2’-deoxyuridine (5-EdU) is a nucleoside analog to thymidine and can be incorporated into DNA during DNA synthesis (see also our EdU cell proliferation assays for more information). The newly synthesized DNA carries an alkyne moiety that can be easily clicked to azides to assess cell proliferation, cell cycles, viability, etc.



    Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry, R. Kumar et al., 2007, J. Am. Chem. Soc., Vol. 129, p. 6859–6864.


    A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective “Ligation” of Azides and Terminal Alkynes, V. V. Rostovtsev et al., 2002, Angew. Chemie Int. Ed., Vol. 41, p. 2596–2599.


    Proliferation and Differentiation of Gastric Mucous Neck and Chief Cells During Homeostasis and Injury-induced Metaplasia, J. Burclaff et al., 2020, Gastroenterology, Vol. 158(3), p. 598-609.


    Click Chemistry Generated Model DNA–Peptide Heteroconjugates as Tools for Mass Spectrometry, F. J. Flett et al., 2015, Analytical Chemistry, Vol. 87(19), p. 9595–9599.


    • Molecular Formula


    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dry

    • Molecular Weight

      252.23 g/mol

    • Purity

      ≥ 98% (HPLC)

    • Physical State

      white to off-white solid (water content < 0.5 % w/w)

    • CAS Number


    • Additional name

      EdU, 2′-Deoxy-5-ethynyluridine, 5-Ethynyl-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4(1H,3H)-dione

    • Solubility

      DMSO, Alcohol, Water, Aqueous buffers