5-Ethynyl-2’-deoxyuridine (5-EdU) is a nucleoside analog to thymidine and can be incorporated into DNA during DNA synthesis (see also our EdU cell proliferation assays for more information). The newly synthesized DNA carries an alkyne moiety that can be easily clicked to azides to assess cell proliferation, cell cycles, viability, etc.

LITERATURE

Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry, R. Kumar et al., 2007, J. Am. Chem. Soc., Vol. 129, p. 6859–6864.

https://doi.org/10.1021/ja070273v

A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective “Ligation” of Azides and Terminal Alkynes, V. V. Rostovtsev et al., 2002, Angew. Chemie Int. Ed., Vol. 41, p. 2596–2599.

https://doi.org/10.1002/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4

Proliferation and Differentiation of Gastric Mucous Neck and Chief Cells During Homeostasis and Injury-induced Metaplasia, J. Burclaff et al., 2020, Gastroenterology, Vol. 158(3), p. 598-609.

https://doi.org/10.1053/j.gastro.2019.09.037

Click Chemistry Generated Model DNA–Peptide Heteroconjugates as Tools for Mass Spectrometry, F. J. Flett et al., 2015, Analytical Chemistry, Vol. 87(19), p. 9595–9599.

https://doi.org/10.1021/acs.analchem.5b02047