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5-Ethynyl uridine (5-EU)

Clickable EU allows detection of de novo RNA

Size Catalog No. Price
5 mg BCN-003-5  40,00
10 mg BCN-003-10  65,00
100 mg BCN-003-100  400,00
  • 5-Ethynyl uridine (5-EU) is a modified nucleoside that can be used for labeling of nascent RNA inside cells. After feeding EU, it can react with azides. Our own experiments indicate that it will be first integrated in de novo synthesized RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications.



    Exploring RNA transcription and turnover in vivo by using click chemistry, C. Y. Jao et al., 2008, Proc. Natl. Acad. Sci. U. S. A., Vol. 105, p. 15779–15784.


    Capturing the interactome of newly transcribed RNA, X. Bao et al., 2018, Nature Methods, Vol. 15, p. 213-220.


    Visualization of the Nucleolus Using Ethynyl Uridine, M. Dvořáčková et al., 2018, Frontiers in Plant Science, Vol. 9(177).


    • Molecular Formula


    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dry, inert gas

    • Molecular Weight

      268.22 g/mol

    • Purity

      ≥ 98% (HPLC)

    • Physical State

      white to light brown to light green powder

    • CAS Number


    • Additional name

      EU, 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione

    • Solubility

      water, aqueous buffer, DMSO, ethanol