We enable nucleic acid labeling bioconjugation
Generic selectors
Exact matches only
Search in title
Search in content
Search in posts
Search in pages
product_variation
show blocks helper

Product Application for...

Product Detetction by

Product Emission color

Product Enatiomeric structure

Product Kits for..

Product Label Position

Product Linker type

Product Metabolit

Product Modification Type

Product Nucleoside

Product Nucleosites

Product Protection Type

Product Sort by

Product Sugar core

5-Ethynyl uridine

Clickable EU allows Detection of de novo RNA

Currency:  
  • Size
  • Catalog No.
  • Price
  • 5.0 mg
  • BCN-003-5

  • € 55,00
  • 10 mg
  • BCN-003-10

  • € 90,00
Clear
  • 5-ethynyl uridine (EU) is a modified nucleoside that can be used for labeling of nascent RNA inside cells. After feeding EU, it can be reacted to fluorescent dye alkynes via click labeling and detected. Our own experiments indicate that it will be first integrated in de novo RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications

    LITERATURE

    C. Y. Jao, A. Salic, Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 15779–15784.

    FAQ

    • Can I use 5-ethynyl uridine (EU) for labeling of nascent RNA inside cells?

      EU can be fed to cells and detected using a fluorescent dye via click labeling. Our own experiments indicate that it will be first integrated in de novo RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications. (1)(2)
      1. Jao,C.Y. and Salic,A. (2008) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Natl. Acad. Sci. U. S. A., 105, 15779–84.
      2. Bao,X., Guo,X., Yin,M., Tariq,M., Lai,Y., Kanwal,S., Zhou,J., Li,N., Lv,Y., Pulido-quetglas,C., et al. (2018) Capturing the interactome of newly transcribed RNA. 15.

    • Molecular Formula

      C11H12N2O6

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dry, inert gas

    • Molecular Weight

      268.22 g/mol

    • Purity

      >98% (HPLC)

    • Physical State

      white to light brown to light green powder

    • CAS Number

      69075-42-9

    • Additional name

      5-EU, EU

    • Solubility

      water, aqueous buffer, DMSO, ethanol