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5-Ethynyl uridine
Clickable EU allows Detection of de novo RNA
- Size
- Catalog No.
- Price
- 5.0 mg
BCN-003-5
- € 40,00
- 10 mg
BCN-003-10
- € 65,00
- 100 mg
BCN-003-100
- € 400,00
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5-ethynyl uridine (EU) is a modified nucleoside that can be used for labeling of nascent RNA inside cells. After feeding EU, it can be reacted to fluorescent dye alkynes via click labeling and detected. Our own experiments indicate that it will be first integrated in de novo RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications
LITERATURE
C. Y. Jao, A. Salic, Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 15779–15784.
FAQ
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Can I use 5-ethynyl uridine (EU) for labeling of nascent RNA inside cells?
EU can be fed to cells and detected using a fluorescent dye via click labeling. Our own experiments indicate that it will be first integrated in de novo RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications. (1)(2)
1. Jao,C.Y. and Salic,A. (2008) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Natl. Acad. Sci. U. S. A., 105, 15779–84.
2. Bao,X., Guo,X., Yin,M., Tariq,M., Lai,Y., Kanwal,S., Zhou,J., Li,N., Lv,Y., Pulido-quetglas,C., et al. (2018) Capturing the interactome of newly transcribed RNA. 15.
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Can I use 5-ethynyl uridine (EU) for labeling of nascent RNA inside cells?
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Molecular Formula
C11H12N2O6
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dry, inert gas
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Molecular Weight
268.22 g/mol
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Purity
≥ 98% (HPLC)
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Physical State
white to light brown to light green powder
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CAS Number
69075-42-9
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Additional name
5-EU, EU
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Solubility
water, aqueous buffer, DMSO, ethanol
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Molecular Formula