Product Application for...
Product Detetction by
Product Emission color
Product Enatiomeric structure
Product Kits for..
Product Label Position
Product Linker type
Product Modification Type
Product Nucleoside
Product Nucleosites
Product Protection Type
Product Sort by
Product Sugar core
5-Ethynyl-UTP
Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Size
- Catalog No.
- Price
- 0.5 µmol
BCT-07-S
- € 150,00
- 2.5 µmol
BCT-07-M
- € 580,00
- 5.0 µmol
BCT-07-L
- € 750,00
-
Our 5′-Ethynyl-UTP can be incorporated into RNA using standard nucleotide mixtures and T7 RNA polymerase guided in-vitro transcription. Ending up with a partially modified RNA (we recommend to replace the natural base by only 1-10% with the modified one), it is ready for labeling with our marker azides (e.g. fluorescent dyes for tracking and imaging or cross-linking to other biomolecules) by click chemistry.
LITERATURE
Presolski et al., Current Protocols of Chemical Biology, 2011, 3:153
Hong et al., Angew. Chem. Int. Ed., 2011, 48:9879
S.Croce, S. Serdjukow, T. Carell, T. Frischmuth, ChemBioChem, 2020, 21, 1-7
-
-
Molecular Formula
C11H15N2O15P3
-
Shelf Life
12 months unopened after receipt
-
Storage Conditions
-20 °C
-
Molecular Weight
507.97 g/mol
-
Purity
≥ 95% (HPLC)
-
Physical State
100 mM clear aquaeous solution; colorless to slightly yellow
-
CAS Number
n.a.
-
Absorption (max)
λmax = 288 nm
-
Ɛ (max)
12,000 cm-1M-1
-
Molecular Formula