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5-Ethynyl-UTP
Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Size
- Catalog No.
- Price
- 0.5 µmol
BCT-07-S
- € 150,00
- 2.5 µmol
BCT-07-M
- € 580,00
- 5.0 µmol
BCT-07-L
- € 750,00
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Our 5′-Ethynyl-UTP can be incorporated into RNA using standard nucleotide mixtures and T7 RNA polymerase guided in-vitro transcription. Ending up with a partially modified RNA (we recommend to replace the natural base by only 1-10% with the modified one), it is ready for labeling with our marker azides (e.g. fluorescent dyes for tracking and imaging or cross-linking to other biomolecules) by click chemistry.
LITERATURE
Presolski et al., Current Protocols of Chemical Biology, 2011, 3:153
Hong et al., Angew. Chem. Int. Ed., 2011, 48:9879
S.Croce, S. Serdjukow, T. Carell, T. Frischmuth, ChemBioChem, 2020, 21, 1-7
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Molecular Formula
C11H15N2O15P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
507.97 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
100 mM clear aquaeous solution; colorless to slightly yellow
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CAS Number
n.a.
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Absorption (max)
λmax = 288 nm
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Ɛ (max)
12,000 cm-1M-1
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Molecular Formula