IVT High Performance T7 RNA Synthesis Kit

Optimized RNA synthesis with the IVT high performance T7 RNA synthesis kit

The IVT High Performance T7 RNA Synthesis Kit by baseclick is a complete, ready-to-use system for synthesizing RNA from linear DNA templates using T7 RNA polymerase. Optimized for both standard and chemically modified nucleotides, this kit delivers high yields of full-length mRNA in high purity and reliable performance across a wide range of research and biotechnology applications.

The included GMP-grade T7 RNA polymerase ensures exceptional purity and consistency, even for sensitive downstream uses such as mRNA therapeutic and vaccine development, RNA labeling, and translation studies. The kit is fully compatible with click-modified nucleotides and supports synthesis of functionalized RNAs without the need for protocol modifications.

Challenges faced by commercial IVT Kits

Many commercial IVT kits suffer from:

• Low enzyme stability
• Poor compatibility with modified nucleotides
• Inconsistent or suboptimal RNA yields, particularly in demanding workflows such as chemical biology, RNA imaging, or mRNA therapeutic development.

These limitations often result in degraded RNA transcripts, batch variability, and inefficient incorporation of specialized nucleotides, especially when functional modifications are required.

Consistent high-yield RNA synthesis with baseclick’s IVT kit

With baseclick’s IVT High Performance T7 RNA Synthesis Kit, you can expect:

• Consistently high RNA yields – up to 180 µg in average per reaction
• Excellent RNA quality and integrity
• Smooth, efficient incorporation of click-compatible and RNA-stabilizing nucleotides
• Reliable, publication-ready results across a variety of experimental applications

Key advantages for Your research needs

• Click-Chemistry Compatible: Seamless incorporation of azide-, alkyne-, or methylated nucleotides, no protocol adjustments required
• GMP-Grade Reagents: Ensures purity, reproducibility, and regulatory-ready performance
• High Efficiency: RNA synthesis from as little as 1 µg DNA template, with optimized yield and minimal inhibition
• Enzyme Stability: Highly concentrated polymerase and pyrophosphatase for longer shelf life and robust activity
• High Quality: Outstanding homogeneity of the full-length RNA product, very low incorporation mistake rate, very few RNA double strands
• Enhanced Yield: Pyrophosphatase shifts the reaction equilibrium, increasing transcription output

Comprehensive kit components for optimal RNA Synthesis

• T7 RNA Polymerase (GMP-grade) – High-performance enzyme for efficient transcription
• Pyrophosphatase (GMP-grade) – Removes inhibitory pyrophosphate to enhance RNA yield
• 5× Transcription Buffer – Optimized formulation for maximum efficiency
• CTP, UTP, GTP, ATP (100 mM each) – Ultra-pure NTPs for precise incorporation
• Linear DNA Control Template (mScarlet) – Ensures batch-to-batch consistency and quality assurance

Technical Data

 Limited mRNA yield is a common bottleneck in many experimental workflows. The IVT High Performance T7 RNA Synthesis Kit by baseclick overcomes this challenge by delivering 140 – 180 µg of mRNA per standard 20 µL reaction, equivalent to up to 8 mg of high-quality, homogeneous mRNA per kit.

mRNA synthesis from PCR products is equally efficient. As little as 200 ng of linear DNA is sufficient to drive a full 20 µL reaction, streamlining template preparation and reducing input requirements.

Figure 1: mRNA yield per IVT kit. Compared to kits from other manufacturers, the IVT High Performance T7 RNA Synthesis Kit from baseclick delivers excellent performance and outstanding RNA product quality.

Most IVT kits are optimized for mRNA transcripts around 2 kb, the average length of spliced eukaryotic mRNAs. However, generating shorter or significantly longer transcripts often results in reduced yields and poor RNA quality.

baseclick’s IVT High Performance T7 RNA Synthesis Kit delivers consistently high yields and excellent RNA integrity across a broad range of transcript lengths. Transcripts as short as 0.5 kb are synthesized with the same efficiency as standard-length mRNAs, and exceptionally long RNAs — up to 10 kb — can be produced with outstanding homogeneity.

Figure 2: Influence of RNA transcript length on IVT Yield: The IVT High Performance T7 RNA Synthesis Kit from baseclick works well with very short transcripts.

Incorporating non-canonical nucleotides remains a key hurdle in IVT workflows, despite their proven benefits for mRNA stability and immunogenicity. baseclick´s IVT High Performance T7 RNA Synthesis Kit enables efficient synthesis of ethynyluridine-modified mRNA with yields exceeding 50 µg per reaction. Reactions with pseudouridine or N1-methylpseudouridine deliver comparable yields to standard UTP-based syntheses, without compromising performance.

Figure 3: The IVT High Performance T7 RNA Synthesis Kit from baseclick is perfectly suited for use with modified RNA building blocks. It allows for the synthesis of large quantities of high-quality, internally modified mRNA.

For in vitro transcribed mRNA to be efficiently translated, a 5′-cap structure mimicking natural eukaryotic mRNA is essential. However, capping can be technically demanding. The IVT High Performance T7 RNA Synthesis Kit by baseclick is optimized for seamless, one-pot synthesis of capped mRNAs using advanced cap analogs such as CleanCap® and ARCA (BCT-38 and BCT-24).

Figure 4: The IVT High Performance T7 RNA Synthesis Kit from baseclick is perfectly suited for co-transcriptional capping. The high-quality mRNA is ready to use for translation.

The result is a highly homogeneous mRNA product with >95 % capping efficiency and yields equivalent to standard, uncapped IVT reactions. Even when incorporating pseudouridine or N1-methylpseudouridine, the synthesis of capped mRNA proceeds efficiently—without compromising yield or quality.

Application versatility for diverse research needs

 Perfectly suited for:

• mRNA synthesis (research and therapeutic)
• RNA modification and click chemistry labeling
• RNA imaging and probe generation
• Synthetic biology and gene expression studies