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Biotin Azide Plus

Improved biotinylation reagent for labeling by CuAAC

Size Catalog No. Price
5 mg BCFA-235-5 290 €
  • Biotin Azide Plus is a biotin probe that enables the possibility for copper-catalyzed Azide-Alkyne Chemistry (CuAAC) to biotin label alkyne or DBCO modified targets. The Azide Plus is a chemical structure that works as a strong copper-chelating system allowing the formation of a strong and active copper complex that makes Biotin Azide Plus the reactant and the catalyst in the CuAAC reaction. Therefore the reaction can be performed under diluted conditions. Once the biotin is attached to a biomolecule fluorescence microscopy detection can be carried out using the well-known signal enhancement strategy of incubation with a fluorescent streptavidin-conjugate.



    Essential requirement for JPT2 in NAADP-evoked Ca2+ signaling, G. S. Gunaratne et al., 2021, Science Signaling, Vol. 14(675), eabd5605.


    TMEM132A, a novel Wnt signaling pathway regulator through Wntless (WLS) interaction, B. Li et al., 2020, Frontiers in Cell and Developmental Biology, Vol. 8, 599890.


    • Molecular Formula


    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark, inert gas

    • Molecular Weight

      582.72 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      off-white to slightly orange amorphous solid or oil

    • CAS Number


    • Solubility

      DMSO, DMF, MeOH

    • Preparation/Handling

      For a 10 mM solution add 858 μL to 5 mg.
      For a 10 mM solution add 1716 μL to 10 mg.