Biotin-PEG4-Azide is a biotin probe that enables the possibility for copper-catalyzed Azide-Alkyne Chemistry (CuAAC) to biotin label alkyne or DBCO modified targets. Once the biotin is attached to a biomolecule, fluorescence microscopy detection can be carried out using the well-known signal enhancement strategy of incubation with a fluorescent streptavidin-conjugate.

The introduction for the hydrophilic PEG4-moity (19 atoms) reduces aggregation and precipitation during the labeling reaction due to an increase in hydrophilicity and also enhances the detection efficiency of the biotin due to reducing sterically hindrance.

LITERATURE

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