This C8-Alkyne-dU-CE Phosphoramidite, containing a C8-Spacer, is used under standard conditions for solid phase synthesis of oligonucleotides. It has shown to be incorporated into DNA oligomers with excellent coupling efficiency.

LITERATURE

Nucleosides And Oligonucleotides With Diynyl Side Chains: The Huisgen-Sharpless Cycloaddition “Click Reaction” Performed On Dna And Their Constituents, F. Seela et al., 2007, Nucleosides, Nucleotides & Nucleic Acids, Vol. 26, p. 597–601.

https://doi.org/10.1080/15257770701490308

DNA-Photographie: eine ultraempfindliche Methode zur Detektion von DNA mithilfe photographischer Techniken, D. M. Hammond et al., 2007, Angew. Chem., Vol. 119, p. 4262–4265.

https://doi.org/10.1002/ange.200605023

Transfer Printing of DNA by “Click” Chemistry, D. I. Rozkiewicz et al., 2007, ChemBioChem, Vol. 8, p. 1997–2002.

https://doi.org/10.1002/cbic.200700402

DNA Containing Side Chains with Terminal Triple Bonds: Base-Pair Stability and Functionalization of Alkynylated Pyrimidines and 7-Deazapurines, F. Seela et al., 2006, Chemistry & Biodiversity, Vol. 3, p. 509–514.

https://doi.org/10.1002/cbdv.200690054

Click Chemistry as a Reliable Method for the High-Density Postsynthetic Functionalization of Alkyne-Modified DNA, J. Gierlich et al., 2006, Org. Lett., Vol. 8, p. 3639–3642.

https://doi.org/10.1021/ol0610946

A click chemistry approach to developing molecularly targeted DNA scissors, T. Lauria et al., 2020, Chemistry – A European Journal, Vol. 26(70), p. 16782-16792.

https://doi.org/10.1002/chem.202002860

Supersensitive Multifluorophore RNA‐FISH for Early Virus Detection and Flow‐FISH by Using Click Chemistry, N. Raddaoui et al., 2020, ChemBioChem, Vol. 21(15), p. 2214-2218.

https://doi.org/10.1002/cbic.202000081

Selective targeting and degradation of doxorubicin-loaded folate-functionalized DNA nanocages, S. Raniolo et al., 2018, Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 14(4), p. 1181-1190.

https://doi.org/10.1016/j.nano.2018.02.002