We enable nucleic acid labeling bioconjugation
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ClickTech PCR Modification Kits

Preparation of fluorescent-labeled DNA products by PCR

Currency:  
  • Size
  • Catalog No.
  • Price
  • Dye 488
  • BCK-PCR-MOD-488

  • 500 €
  • Dye 555
  • BCK-PCR-MOD-555

  • 500 €
  • Dye 594
  • BCK-PCR-MOD-594

  • 500 €
  • Dye 647
  • BCK-PCR-MOD-647

  • 500 €
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  • baseclick’s ClickTech PCR Modification Kits contain all reagents required for generating highly labelled PCR products via an easy-to-handle two-step method based on click chemistry.

    The Mixture of unmodified dNTP and alkyne modified- (EdUTP) nucleotides can be used in PCR reactions with the optimized baseclick Ethynyl Polymerase. The resulting PCR products contain alkyne moieties, which react with azido-derivatives of fluorescent dyes, haptnes and other labels in a highly selective fashion under benign click reaction conditions. We offer within this kit click reagents and a clickable dye of your choice (green 488, yellow 555, orange 594 or red 647) obtaining fluorescent labeled PCR products.

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      – 20 °C

    • Physical State

      kit system made of different components

    • CAS Number

      n.a.

    • Excitation (max)

      Dye 488: 496 nm | Dye 555: 546 nm | Dye 594: 584 nm | Dye 647: 643 nm

    • Emission (max)

      Dye 488: 516 nm | Dye 555: 579 nm | Dye 594: 603 nm | Dye 647: 662 nm

    • Ɛ (max)

      Dye 488: 83.000 cm-1M-1 | Dye 555: 91.000 cm-1M-1 | Dye 594: 110.000 cm-1M-1 | Dye 647: 250.000 cm-1M-1

    • Preparation/Handling

      please see user manual of the kit

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