We enable nucleic acid labeling bioconjugation
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5-Ethynyl-dUTP (5-EdUTP)

Modified triphosphate for incorporation in PCR reaction

  • Size
  • Catalog No.
  • Price
  • 1 µmol
  • BCT-08-S

  • € 100,00
  • 5 µmol
  • BCT-08-L

  • € 320,00
  • 5-Ethynyl-dUTP (5-EdUTP) is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dUTP.


    Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. Eur. J., Vol. 13(34), p. 9486–9494.


    Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128(5), p. 1398–1399.


    Identification and characterization of nucleobase-modified aptamers by click-SELEX, F. Pfeiffer et al., 2018, Nature Protocols, Vol. 13(5), p. 1153–1180.


    Quantitation of deoxynucleoside triphosphates by click reactions, C. Y. Huang et al., 2020Scientific Reports, Vol. 10(1), p. 611.


    • Molecular Formula


    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      492.16 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      100 mM clear colorless solution in water (pH 7.5)

    • CAS Number


    • Additional name

      EdUTP; 5-Ethynyl-2’-deoxyuridine-5’-triphosphate

    • Absorption (max)

      λmax = 288 nm

    • Ɛ (max)

      12,000 cm-1M-1