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5-Ethynyl-dUTP (5-EdUTP)

Modified triphosphate for incorporation in PCR reaction

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1 µmol
  • BCT-08-S

  • € 120,00
  • 5 µmol
  • BCT-08-L

  • € 380,00
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  • 5-Ethynyl-dUTP (5-EdUTP) is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dUTP.

     

    LITERATURE

    Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. Eur. J., Vol. 13(34), p. 9486–9494.

    https://doi.org/10.1002/chem.200700502

    Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128(5), p. 1398–1399.

    https://doi.org/10.1021/ja055517v

    Identification and characterization of nucleobase-modified aptamers by click-SELEX, F. Pfeiffer et al., 2018, Nature Protocols, Vol. 13(5), p. 1153–1180.

    https://doi.org/10.1038/nprot.2018.023

    Quantitation of deoxynucleoside triphosphates by click reactions, C. Y. Huang et al., 2020Scientific Reports, Vol. 10(1), p. 611.

    https://doi.org/10.1038/s41598-020-57463-3

    • Molecular Formula

      C11H15N2O14P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      492.16 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      100 mM clear colorless solution in water (pH 7.5)

    • CAS Number

      111289-87-3

    • Additional name

      EdUTP; 5-Ethynyl-2’-deoxyuridine-5’-triphosphate

    • Absorption (max)

      λmax = 288 nm

    • Ɛ (max)

      12,000 cm-1M-1

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