We enable nucleic acid labeling bioconjugation
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DBCO-PEG4-Desthiobiotin

Desthiobiotinylation reagent for labeling by SPAAC

Currency:  
  • Size
  • Catalog No.
  • Price
  • 5 mg
  • BCFA-233-5

  • € 140,00
  • 10 mg
  • BCFA-233-10

  • € 260,00
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  • DBCO-PEG4-Desthiobiotin can be incorporated to biomolecules via strain-promoted Alkyne-Azide Chemistry (SPAAC) which does not need a Cu (I) catalysis or heating. Desthiobiotin binds less tightly to streptavidin than biotin and therefore Desthiobiotin labelled biomolecules can easily be eluted from streptavidin affinity resin by using free biotin.

    The introduction of the hydrophilic PEG4-moity reduces aggregation and precipitation during the labeling reaction by increasing the hydrophilicity.

     

    LITERATURE

    Quantitative secretome analysis of activated Jurkat cells using click chemistry-based enrichment of secreted glycoproteins, K. E. Witzke et al., 2017, Journal of Proteome Research, Vol. 16(1), p. 137-146.

    https://doi.org/10.1021/acs.jproteome.6b00575

    Tetracyclines modify translation by targeting key human rRNA substructures, J. D. Mortison et al., 2018, Cell chemical biology, Vol. 25(12), p. 1506-1518.

    https://doi.org/10.1016/j.chembiol.2018.09.010

    • Molecular Formula

      C39H53N5O8

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      719.87 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      white to yellow solid

    • CAS Number

      2032788-37-5

    • Solubility

      DMSO, DMF, MeOH, DCM, THF

    • Preparation/Handling

      For a 10 mM solution add 695 μL to 5 mg.
      For a 10 mM solution add 1389 μL to 10 mg.

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