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Eterneon-Red 645-Picolyl-Azide

Improved 647 dye to label with reduced copper concentrations

Size Catalog No. Price
1 mg BCFA-248-1  250,00
5 mg BCFA-248-5  750,00
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  • This special red-fluorescent dye enables advanced click reaction kinetics due to the usage of a picolyl-azide. This structure can complex copper and, therefore, the dye is already connected to its catalyst for the efficient click reaction. One benefit is that reducing copper concentration allows click reaction with sensitive reactants. Picolyl azides enhance the reaction kinetics so much that it is possible to have a picolyl structure that reacts selectively with an alkyne even when other azides are present.[1]

    This enables among other things the usage of less amount of copper in the click reaction mixture without any effect on the great reaction efficiency achieving the same reaction yields. This effect can be crucial e.g. when copper sensitive enzyme or biomolecules shall be labeled. Once attached to a biomolecule via click chemistry (CuAAC, copper catalyzed alkyne-azide cycloaddition) fluorescence microscopy detection can be performed similar to standard dyes. Due to the spectral properties the Eterneon-Red 645 picolyl dye can be used as AF647 alternative and Atto 647 alternative.

     

    LITERATURE

    [1] Efficient Tandem Copper‐Catalyzed Click Synthesis of Multisugar‐Modified Oligonucleotides. Angewandte Chemie International Edition, e202405161.

    https://doi.org/10.1002/anie.202405161

    • Molecular Formula

      C43H52N8O11S3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      953.15 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      blue solid

    • CAS Number

      n.a.

    • Excitation (max)

      647 nm

    • Emission (max)

      663 nm

    • Ɛ (max)

      225.000 cm-1M-1

    • Solubility

      Water, DMSO, DMF, MeOH, EtOH

    • Preparation/Handling

      For a 10 mM solution add 104 μL to 1 mg.
      For a 10 mM solution add 520 μL to 5 mg.

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