DBCO-AF647
Dye to label by SPAAC in copper-free environment
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This special red fluorescent dye DBCO-AF647 enables the possibility for copper-free click chemistry which is mostly used in reaction where cytotoxic copper catalyst is not an option. The click reaction takes place via strain-promoted Alkyne-Azide Chemistry (SPAAC) which does not need a Cu (I) catalysis or heating. Once attached to a biomolecule, fluorescence microscopy detection can be carried out just like with Cy5, or any other alternative dye.
LITERATURE
Chemoselective Bioconjugation of Amyloidogenic Protein Antigens to PEGylated Microspheres Enables Detection of α-Synuclein Autoantibodies in Human Plasma, P. Ge et al., 2022, Bioconjugate Chemistry, Vol. 33(2), p. 301-310.
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Molecular Formula
C55H62N4O14S4
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
1117.33 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
blue solid
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CAS Number
n.a.
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Excitation (max)
648 nm
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Emission (max)
671 nm
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Ɛ (max)
270.000 cm-1M-1
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Solubility
H2O, DMSO, DMF
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Preparation/Handling
For a 10 mM solution add 89.5 μL to 1 mg.
For a 10 mM solution add 447.5 μL to 5 mg.
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Molecular Formula