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Modified triphosphate for stabilizing mRNA

Size Catalog No. Price
1 µmol BCT-37-S € 270,00
5 µmol BCT-37-L € 1.050,00
  • N1-methylpseudo-UTP is a modified nucleotide, used for synthesizing mRNA with reduced immunogenicity and improved stability. It was reported that N1-methylpseudouridine (m1Ψ) modification alone or in combination with 5-methylcytidine (m5C) exhibited superiority over pseudouridine (Ψ) or m5C/Ψ-modified mRNA platform by providing up to ~ 44-fold (when comparing double modified mRNAs) and ~ 13-fold (when comparing single modified mRNAs) higher reporter gene expression in mammalian cells and mice, respectively. Moreover, compared with (m5C/)Ψ-modified mRNAs, (m5C/)m1Ψ-modified mRNAs showed reduced intracellular innate immunogenicity and resulted in improved cellular viability after in vitro transfection.[1] Therefore, N1-methylpseudouridine Triphosphate can serve as a powerful ingredient for synthesizing new mRNA drugs with better performance.



    [1] N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice, O. Andries et al., 2015, Journal of Controlled Release, Vol. 217, p. 337-344.


    Multicomponent Lipid Nanoparticles for RNA Transfection, N. Gretskaya et al., 2023, Pharmaceutics, Vol. 15(4), 1289.


    CpG oligodeoxynucleotide developed to activate primate immune responses promotes antitumoral effects in combination with a neoantigen-based mRNA cancer vaccine, Q. Li et al., 2021, Drug Design, Development and Therapy, p. 3953-3963.


    • Molecular Formula

      C10H17N2O15P3 (free acid)

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark, dry

    • Molecular Weight

      498.10 g/mol (free acid)

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      colorless solution (100 mM, pH 7.5)

    • CAS Number


    • Absorption (max)

      λmax = 271 nm

    • Ɛ (max)

      8877 cm-1M-1