Control your RNA functions through defined modification, functionalization, and targeting strategies
Integrated baseclick × Eurofins Genomics workflow
All steps are coordinated within a single expert‑led service, from template handling to functional RNA.
Defined RNA modification and functionalization
Select post‑transcriptional modification, labeling, and targeting options suited to your application.
Custom mRNA synthesis and RNA modification services for research and preclinical applications
Choose your RNA Application
Transient protein expression
Expression levels are often affected by RNA stability, cap structure, and nucleotide composition, leading to variability.
baseclick controls these parameters at the RNA design stage to improve stability and translation consistency.
RNA labeling and tracking
Labeling of RNA for visualization of localization, uptake, and intracellular distribution.
Variability often arises from unspecific labeling or interference with RNA function.
baseclick enables site‑specific functionalization of RNA without affecting sequence integrity.
Targeted RNA Delivery
Design of RNA constructs for receptor-mediated cellular targeting. Standard delivery systems often lack specificity and control over RNA–cell interactions. baseclick enables site-specific conjugation of targeting ligands such as GalNAc, mannose, peptides, and antibodies to create defined targeted RNA delivery systems.
RNA Uptake & Intracellular Trafficking Studies
Evaluation of cellular uptake pathways, endosomal escape and intracellular trafficking of RNA constructs. baseclick provides chemically defined and site-specifically modified RNA constructs to investigate uptake and intracellular transport independently of delivery formulations.
RNA modification screening
Systematic evaluation of RNA design parameters on stability and translation.
Screening approaches are often inconsistent due to uncontrolled variation of RNA features.
baseclick allows controlled variation of cap structures, nucleotides, and RNA composition.
RNA stability studies
Analysis of RNA stability in biological systems.
Rapid degradation and undefined RNA composition limit reproducibility.
baseclick enables defined incorporation of stabilizing modifications to control RNA persistence.
Gene regulation studies (siRNA / mRNA)
siRNA enables sequence-specific suppression of target genes, while mRNA enables controlled expression of proteins of interest. baseclick provides custom-designed and chemically modified RNA constructs to support reproducible gene regulation studies across a wide range of research applications.
RNA structure & function studies
Analysis of how RNA structure affects biological function.
Standard approaches provide limited control over structural features and modification sites.
baseclick enables site‑specific modification to study defined structure–function relationships.
Synthesis Workflow
1. Input Options
Flexible starting points
Begin your project from sequence, plasmid DNA, or PCR product, depending on your starting material.
Start at sequence level
- DNA template design and plasmid preparation are coordinated with Eurofins Genomics.
- Ideal if no IVT template is available.
Start with plasmid DNA
- Direct proceeds to IVT, including optional post transcriptional modification.
- Best for ongoing research and optimization studies.
Start with PCR product
- PCR products are assessed and converted into IVT compatible templates.
- Suitable for rapid iteration or when plasmid templates are not available.
2. Synthesis Options
Template Synthesis
Full process from DNA template to application-ready RNA.
Our capabilities include:
- Codon and UTR optimization to support protein expression
- Poly(A) tail options, such as definded-length tails, custom tails, or no poly(A) tail
- DNA plasmid linearization
- Learn more
Transcriptional RNA Synthesis
RNA properties can be adjusted directly during IVT through defined co-transcriptional modifications, including:
- non-capped
- 5′-capping strategies (e.g. Cap0, Cap1, and modified capping initiators)
- Incorporation of modified nucleotides during transcription
- Learn more
RNA Functionalization
Introduction of additional functionalities after transcription using site-specific and internal chemical modification approaches, including:
- Controlled attachment of fluorophores or affinity tags
- Introduction of targeting ligands (e.g. sugars, peptides, fatty acids)
- Enzymatic synthesis or modification of Poly A tail
- Learn more
Order Process Workflow
When to choose baseclick
Our platform is designed for researchers who require functional, reproducible RNA with defined performance, not just synthesis, for:
- RNA therapeutics and preclinical research
- Targeted delivery and uptake studies
- RNA labeling, imaging, and tracking
- Functional RNA assays
What you gain
- Patented click chemistry for precise RNA functionalization
- Validated workflows across mRNA, siRNA, LNA, and DNA
- Improved stability and translation performance
- Proven targeting strategies (e.g. GalNAc, mannose)