2-Ethynyl-ATP (2-EATP)
Triphosphate for modifying of 3'END RNA
| Size | Catalog No. | Price |
|---|---|---|
| 1 µmol | BCT-30-S | € 190,00 |
| 5 µmol | BCT-30-L | € 720,00 |
Chemical Properties
-
Molecular Formula
C12H16N5O13P3
-
Shelf Life
12 months unopened after receipt
-
Storage Conditions
-20 °C, dark, dry
-
Molecular Weight
531.20 g/mol
-
Purity
≥ 90% (HPLC)
-
Physical State
colorless to slightly yellow solution (10 mM)
-
CAS Number
1802333-53-4
-
Absorption (max)
λmax = 265 nm
-
Ɛ (max)
10.600 cm-1M-1
Product Information
2-Ethynyl-ATP (2-EATP) is a modified nucleotide that is commonly used in enzymatic assays intended to analyse the binding of ATP to proteins or it can be used to investigate ATP-depended enzymes in various pathological conditions. 2-EATP is also suitable for in vitro polyadenylation of natural RNA by recombinant poly(A) polymerase. The click reaction can be performed under the same conditions as before.
LITERATURE
Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures, F. Chen et al., 2019, Nucleic Acids Research, Vol. 47(22), p. e145.
https://doi.org/10.1093/nar/gkz852
Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap, D. Curanovic et al., 2013, Nature Chemical Biology, Vol. 9, p. 671–673.
https://doi.org/10.1038/nchembio.1334
A Platform for Controlled Template-Independent Enzymatic Synthesis of RNA Oligonucleotides and Therapeutics, D. J. Wiegand et al., 2023, bioRxiv, 2023-06.