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5-Carboxytetramethylrhodamine-PEG3-Azide (5-TAMRA-PEG3-Azide)

Fluorescent dye to label DNA/RNA

Size Catalog No. Price
5 mg BCFA-037-5 € 80,00
10 mg BCFA-037-10 € 125,00
100 mg BCFA-037-100 € 500,00
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  • 5-Carboxytetramethylrhodamine-PEG3-Azide (5-TAMRA-PEG3-Azide) has the same properties as 5-TAMRA-azide, but has a PEG3 spacer that separates the dye core from the attachable biomolecule and thus minimizes possible interactions between both.

     

    LITERATURE

    Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.

    https://doi.org/10.1002/anie.200802077

    Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.

    https://doi.org/10.1002/anie.200705664

    A chemical method for fast and sensitive detection of DNA synthesis in vivo, A. Salic et al., 2008, Proc. Natl. Acad. Sci. U. S. A., Vol. 105, p. 2415–2420.

    https://doi.org/10.1073/pnas.0712168105

    Chemoenzymatic Preparation of Functional Click-Labeled Messenger RNA, S. Croce et al., 2020, ChemBioChem, Vol. 21(11), p. 1641-1646.

    https://doi.org/10.1002/cbic.201900718

    Synthesis and biological evaluation of anthracene-9,10 dione derivatives as CK2 inhibitors, P. López-Rojas et al., 2023, Results in Chemistry, Vol. 6, p. 100997.

    https://doi.org/10.1016/j.rechem.2023.100997

    • Molecular Formula

      C33H38N6O7

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      630.69 g/mol

    • Purity

      ≥ 90% (LCMS)

    • Physical State

      pink to dark red solid

    • CAS Number

      1380486-02-1

    • Additional name

      5-Carboxytetramethylrhodamine-PEG3-Azide

    • Excitation (max)

      DMF or DMSO: 546 nm

    • Emission (max)

      DMF or DMSO: 579 nm

    • Ɛ (max)

      91,000 cm-1M-1

    • Solubility

      DMSO, DMF, MeOH, slightly soluble in water

    • Preparation/Handling

      For a 10 mM solution add 158 μL to 1 mg.

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