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5-Carboxytetramethylrhodamine-PEG3-Azide (5-TAMRA-PEG3-Azide)
Fluorescent dye to label DNA/RNA via click chemistry
- Size
- Catalog No.
- Price
- 5 mg
BCFA-037-5
- € 80,00
- 10 mg
BCFA-037-10
- € 125,00
- 100 mg
BCFA-037-100
- € 500,00
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5-Carboxytetramethylrhodamine-PEG3-Azide (5-TAMRA-PEG3-Azide) has the same properties as 5-TAMRA-azide, but has a PEG3 spacer that separates the dye core from the attachable biomolecule and thus minimizes possible interactions between both.
LITERATURE
Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.
https://doi.org/10.1002/anie.200802077
Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.
https://doi.org/10.1002/anie.200705664
A chemical method for fast and sensitive detection of DNA synthesis in vivo, A. Salic et al., 2008, Proc. Natl. Acad. Sci. U. S. A., Vol. 105, p. 2415–2420.
https://doi.org/10.1073/pnas.0712168105
Chemoenzymatic Preparation of Functional Click-Labeled Messenger RNA, S. Croce et al., 2020, ChemBioChem, Vol. 21(11), p. 1641-1646.
https://doi.org/10.1002/cbic.201900718
Synthesis and biological evaluation of anthracene-9,10 dione derivatives as CK2 inhibitors, P. López-Rojas et al., 2023, Results in Chemistry, Vol. 6, p. 100997.
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Molecular Formula
C33H38N6O7
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
630.69 g/mol
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Purity
≥ 90% (LCMS)
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Physical State
pink to dark red solid
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CAS Number
1380486-02-1
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Additional name
5-Carboxytetramethylrhodamine-PEG3-Azide
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Excitation (max)
DMF or DMSO: 546 nm
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Emission (max)
DMF or DMSO: 579 nm
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Ɛ (max)
91,000 cm-1M-1
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Solubility
DMSO, DMF, MeOH, slightly soluble in water
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Preparation/Handling
For a 10 mM solution add 158 μL to 1 mg.
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Molecular Formula