5-Ethynyl-dUTP (5-EdUTP)
Modified triphosphate for incorporation in PCR reaction
| Size | Catalog No. | Price |
|---|---|---|
| 1 µmol | BCT-08-S | € 120,00 |
| 5 µmol | BCT-08-L | € 380,00 |
Chemical Properties
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Molecular Formula
C11H15N2O14P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
492.16 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
100 mM clear colorless solution in water (pH 7.5)
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CAS Number
111289-87-3
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Additional name
EdUTP; 5-Ethynyl-2’-deoxyuridine-5’-triphosphate
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Absorption (max)
λmax = 288 nm
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Ɛ (max)
12,000 cm-1M-1
Product Information
5-Ethynyl-dUTP (5-EdUTP) is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by click chemistry. We recommend a substitution of 1 – 10% of dUTP.
LITERATURE
Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. Eur. J., Vol. 13(34), p. 9486–9494.
https://doi.org/10.1002/chem.200700502
Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128(5), p. 1398–1399.
https://doi.org/10.1021/ja055517v
Identification and characterization of nucleobase-modified aptamers by click-SELEX, F. Pfeiffer et al., 2018, Nature Protocols, Vol. 13(5), p. 1153–1180.
https://doi.org/10.1038/nprot.2018.023
Quantitation of deoxynucleoside triphosphates by click reactions, C. Y. Huang et al., 2020, Scientific Reports, Vol. 10(1), p. 611.