AZDye 488 Azide Plus
Improved 488 dye to label with reduced copper concentrations
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This special green-fluorescent dye enables advanced click reaction kinetics due to molecular structure that works as a strong copper-chelating system allowing the formation of a strong and active copper complex, that makes AZDye 488 Azide Plus the reactant and the catalyst in the CuAAC reaction. Therefore, less amount of copper can be used achieving the same reaction efficiency. This effect can be crucial e.g. when copper sensitive enzyme or biomolecules shall be labeled. Once clicked to a biomolecule fluorescence detection is identical to FAM, FITC, or any other alternative dye.
LITERATURE
Engineering of cell-surface receptors for analysis of receptor internalization and detection of receptor-specific glycosylation, C. H. Lee et al., 2024, Chem. Sci., Vol. 15(2), p. 555-565.
https://doi.org/10.1039/D3SC05054H
In-depth immunometabolic profiling by measuring single-cell protein translation inhibition via bioorthogonal noncanonical amino acid tagging (SCENITH-BONCAT), F. Vrieling et al., 2023, bioRxiv, 2023-08.
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Molecular Formula
C29H30N10O10S2
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
754.75 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
orange to red solid
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CAS Number
n.a.
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Excitation (max)
495 nm
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Emission (max)
517 nm
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Ɛ (max)
73.000 cm-1M-1
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Solubility
H2O, DMSO, DMF
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Preparation/Handling
For a 10 mM solution add 132 μL to 1 mg.
For a 10 mM solution add 662 μL to 5 mg.
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Molecular Formula