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Fluorescein Azide (6-FAM-Azide)
FITC - Fluorescent dye to label DNA/RNA
- Size
- Catalog No.
- Price
- 1 mg
BCFA-001-1
- € 45,00
- 5 mg
BCFA-001-5
- € 80,00
- 10 mg
BCFA-001-10
- € 125,00
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Fluorescein Azide (6-FAM-Azide) is a fluorescent dye that allows efficient labeling of biomolecules by click reactions. With identical spectral properties to FITC (Fluorescein-5,6-isothiocyanate), ATTO488, Alexa Fluor 488 or DyLight 488 and alike, it is well suited for most fluorescent detection methods.
LITERATURE
Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.
https://doi.org/10.1002/anie.200802077
Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.
https://doi.org/10.1002/anie.200705664
Synthesis of hemicyanine dyes for ‘click’ bioconjugation, W. H. Zhan et al., 2005, Tetrahedron Lett., Vol. 46, p. 1691–1695.
https://doi.org/10.1016/j.tetlet.2005.01.066
DNA visualization in single molecule studies carried out with optical tweezers: Covalent versus non-covalent attachment of fluorophores, S. Suei et al., 2015, Biochemical and Biophysical Research Communications, Vol. 466(2), p. 226-231.
https://doi.org/10.1016/j.bbrc.2015.09.013
Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis, L. Aliyeva-Schnorr et al., 2016, Chromosome Research, Vol. 24, p. 231–242.
https://doi.org/10.1007/s10577-016-9518-8
Cohesin depleted cells rebuild functional nuclear compartments after endomitosis, M. Cremer et al., 2020, Nature Communications, Vol. 11, p. 6146.
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Molecular Formula
C24H18N4O6
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
458.43 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
yellow to orange solid
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CAS Number
1386385-76-7
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Additional name
6-FAM azide; 6-Carboxyfluoresceine Azide, 4-((3-Azidopropyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid; N-(3-azidopropyl)-3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxamide
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Excitation (max)
50 mM PBS, pH 9: 496 nm
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Emission (max)
50 mM PBS, pH 9: 516 nm
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Ɛ (max)
50 mM PBS, pH 9: 83,000 cm-1M-1
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Solubility
DMSO, DMF, MeOH
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Preparation/Handling
For a 10 mM solution add 218 μL to 1 mg.
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Molecular Formula