We enable nucleic acid labeling bioconjugation
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Fluorescein Azide (6-FAM-Azide)

FITC - Fluorescent dye to label DNA/RNA

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1 mg
  • BCFA-001-1

  • € 45,00
  • 5 mg
  • BCFA-001-5

  • € 80,00
  • 10 mg
  • BCFA-001-10

  • € 115,00
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  • Fluorescein Azide (6-FAM-Azide) is a fluorescent dye that allows efficient labeling of biomolecules by click reactions. With identical spectral properties to FITC (Fluorescein-5,6-isothiocyanate), ATTO488, Alexa Fluor 488 or DyLight 488 and alike, it is well suited for most fluorescent detection methods.

     

    LITERATURE

    Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.

    https://doi.org/10.1002/anie.200802077

    Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.

    https://doi.org/10.1002/anie.200705664

    Synthesis of hemicyanine dyes for ‘click’ bioconjugation, W. H. Zhan et al., 2005, Tetrahedron Lett., Vol. 46, p. 1691–1695.

    https://doi.org/10.1016/j.tetlet.2005.01.066

    DNA visualization in single molecule studies carried out with optical tweezers: Covalent versus non-covalent attachment of fluorophores, S. Suei et al., 2015, Biochemical and Biophysical Research Communications, Vol. 466(2), p. 226-231.

    https://doi.org/10.1016/j.bbrc.2015.09.013

    Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis, L. Aliyeva-Schnorr et al., 2016, Chromosome Research, Vol. 24, p. 231–242.

    https://doi.org/10.1007/s10577-016-9518-8

    Cohesin depleted cells rebuild functional nuclear compartments after endomitosis, M. Cremer et al., 2020, Nature Communications, Vol. 11, p. 6146.

    https://doi.org/10.5061/dryad.vt4b8gtqb

    • Molecular Formula

      C24H18N4O6

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      458.43 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      yellow to orange solid

    • CAS Number

      1386385-76-7

    • Additional name

      6-FAM azide; 6-Carboxyfluoresceine Azide, 4-((3-Azidopropyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid; N-(3-azidopropyl)-3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxamide

    • Excitation (max)

      50 mM PBS, pH 9: 496 nm

    • Emission (max)

      50 mM PBS, pH 9: 516 nm

    • Ɛ (max)

      50 mM PBS, pH 9: 83,000 cm-1M-1

    • Solubility

      DMSO, DMF, MeOH

    • Preparation/Handling

      For a 10 mM solution add 218 μL to 1 mg.

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