This green-fluorescent DBCO-AF488 dye enables the possibility for copper-free click chemistry which is mostly used in reaction where cytotoxic copper catalyst is not an option. The click reaction takes place via strain-promoted Alkyne-Azide Chemistry (SPAAC) which does not need a Cu (I) catalysis or heating. Once clicked to a biomolecule fluorescence detection is identical to FAM, FITC, or any other alternative dye.

LITERATURE

A metabolic labeling way to in situ fabricate bacterial FRET Platform for innate immune defence molecule, Z. Zhang et al., 2022, Sensors and Actuators B: Chemical, Vol. 350, 130913.

https://doi.org/10.1016/j.snb.2021.130913

Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes, H. M. Micek et al., 2023, APL Bioengineering, Vol. 7(1), 016111.

https://doi.org/10.1063/5.0132254

Investigating Peptidoglycan Recycling Pathways in Tannerella forsythia with N-Acetylmuramic Acid Bioorthogonal Probes, K. A. Wodzanowski et al., 2022, ACS Infect. Dis., Vol. 8(9), p. 1831–1838.

https://doi.org/10.1021/acsinfecdis.2c00333