C8-Alkyne-dCTP
Modified triphosphate for incorporation in PCR reaction
| Size | Catalog No. | Price |
|---|---|---|
| 1 µmol | BCT-06-S | € 100,00 |
| 5 µmol | BCT-06-L | € 300,00 |
Chemical Properties
-
Molecular Formula
C17H24N3O13P3
-
Shelf Life
12 months unopened after receipt
-
Storage Conditions
-20 °C
-
Molecular Weight
571.31 g/mol
-
Purity
≥ 95% (HPLC)
-
Physical State
100 mM solution in water (pH 7.5); clear colorless to light yellow solution
-
CAS Number
1374991-80-6 (sodium salt)
1004297-66-8 (free acid)
-
Absorption (max)
λmax = 295 nm
-
Ɛ (max)
9,500 cm-1M-1
Product Information
C8-Alkyne-dCTP is easily incorporated into DNA by PCR instead of dCTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by click chemistry. We recommend a substitution of 1 – 10% of dCTP.
LITERATURE
Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. – A Eur. J., Vol. 13, p. 9486–9494.
https://doi.org/10.1002/chem.200700502
Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128, p. 1398–1399.
https://doi.org/10.1021/ja055517v
Quantitation of deoxynucleoside triphosphates by click reactions, C. Y. Huang et al., 2020, Scientific Reports, Vol. 10(1), p. 611.