We enable nucleic acid labeling bioconjugation
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C8-Alkyne-dCTP

Modified triphosphate for incorporation in PCR reaction

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1 µmol
  • BCT-06-S

  • € 85,00
  • 5 µmol
  • BCT-06-L

  • € 260,00
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  • C8-Alkyne-dCTP is easily incorporated into DNA by PCR instead of dCTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dCTP.

    LITERATURE

    Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. – A Eur. J., Vol. 13, p. 9486–9494.

    https://doi.org/10.1002/chem.200700502

    Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128, p. 1398–1399.

    https://doi.org/10.1021/ja055517v

    Quantitation of deoxynucleoside triphosphates by click reactions, C. Y. Huang et al., 2020, Scientific Reports, Vol. 10(1), p. 611.

    https://doi.org/10.1038/s41598-020-57463-3

    • Molecular Formula

      C17H24N3O13P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      571.31 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      100 mM solution in water (pH 7.5); clear colorless to light yellow solution

    • CAS Number

      1374991-80-6 (sodium salt)

      1004297-66-8 (free acid)

    • Absorption (max)

      λmax = 295 nm

    • Ɛ (max)

      9,500 cm-1M-1

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