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C8-Alkyne-dCTP
Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Size
- Catalog No.
- Price
- 1.0 µmol
BCT-06-S
- € 85,00
- 5.0 µmol
BCT-06-L
- € 260,00
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This modified nucleotide triphosphate can be used in standard nucleotide mixtures to label your PCR-fragments with our marker azides. The incorporation of clickable C8-Alkyne dNTPs is accepted by broad polymerase (e.g. with Pwo, Deep Vent exo- or KOD XL) in PCR. As the alkyne introduction as well as the following labeling step by click chemistry can be so effective, we recommend to not replace the natural base by 100% with the modified one in order not to alter the properties of your PCR fragment too much.
LITERATURE
J. Gierlich, K. Gutsmiedl, P. M. E. Gramlich, A. Schmidt, G. A. Burley, T. Carell, Chem. – A Eur. J. 2007, 13, 9486–9494.
G. A. Burley, J. Gierlich, M. R. Mofid, H. Nir, S. Tal, Y. Eichen, T. Carell, J. Am. Chem. Soc. 2006, 128, 1398–1399.
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Molecular Formula
C17H24N3O13P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
571.31 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
100 mM solution in water (pH 7.5); clear colorless to light yellow solution
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CAS Number
1374991-80-6 (sodium salt)
1004297-66-8 (free acid)
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Absorption (max)
λmax = 295 nm
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Ɛ (max)
9,500 cm-1M-1
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Molecular Formula