Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides
- Catalog No.
- 1.0 µmol
- € 85,00
- 5.0 µmol
- € 260,00
This modified nucleotide triphosphate can be used in standard nucleotide mixtures to label your PCR-fragments with our marker azides. The incorporation of clickable C8-Alkyne dNTPs is accepted by broad polymerase (e.g. with Pwo, Deep Vent exo- or KOD XL) in PCR. As the alkyne introduction as well as the following labeling step by click chemistry can be so effective, we recommend to not replace the natural base by 100% with the modified one in order not to alter the properties of your PCR fragment too much.
J. Gierlich, K. Gutsmiedl, P. M. E. Gramlich, A. Schmidt, G. A. Burley, T. Carell, Chem. – A Eur. J. 2007, 13, 9486–9494.
G. A. Burley, J. Gierlich, M. R. Mofid, H. Nir, S. Tal, Y. Eichen, T. Carell, J. Am. Chem. Soc. 2006, 128, 1398–1399.
12 months unopened after receipt
≥ 95 % (HPLC)
100 mM solution in water (pH 7.5); clear colorless to light yellow solution
1374991-80-6 (sodium salt)
1004297-66-8 (free acid)
λmax = 295 nm
- Molecular Formula