Modified triphosphate for incorporation in PCR reaction
- Catalog No.
- 1 µmol
- € 85,00
- 5 µmol
- € 260,00
C8-Alkyne-dCTP is easily incorporated into DNA by PCR instead of dCTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dCTP.
Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. – A Eur. J., Vol. 13, p. 9486–9494.
Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128, p. 1398–1399.
Quantitation of deoxynucleoside triphosphates by click reactions, C. Y. Huang et al., 2020, Scientific Reports, Vol. 10(1), p. 611.
12 months unopened after receipt
≥ 95% (HPLC)
100 mM solution in water (pH 7.5); clear colorless to light yellow solution
1374991-80-6 (sodium salt)
1004297-66-8 (free acid)
λmax = 295 nm
- Molecular Formula