Product Application for...
Product Emission color
Product Enatiomeric structure
Product Kits for..
Product Label Position
Product Linker type
Product Modification Type
Product Nucleoside
Product Nucleosites
Product Protection Type
Product Sort by
Product Sugar core
C8-Alkyne-dCTP
Modified triphosphate for incorporation in PCR reaction
- Size
- Catalog No.
- Price
- 1 µmol
BCT-06-S
- € 85,00
- 5 µmol
BCT-06-L
- € 260,00
-
C8-Alkyne-dCTP is easily incorporated into DNA by PCR instead of dCTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dCTP.
LITERATURE
Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. – A Eur. J., Vol. 13, p. 9486–9494.
https://doi.org/10.1002/chem.200700502
Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128, p. 1398–1399.
https://doi.org/10.1021/ja055517v
Quantitation of deoxynucleoside triphosphates by click reactions, C. Y. Huang et al., 2020, Scientific Reports, Vol. 10(1), p. 611.
-
-
Molecular Formula
C17H24N3O13P3
-
Shelf Life
12 months unopened after receipt
-
Storage Conditions
-20 °C
-
Molecular Weight
571.31 g/mol
-
Purity
≥ 95% (HPLC)
-
Physical State
100 mM solution in water (pH 7.5); clear colorless to light yellow solution
-
CAS Number
1374991-80-6 (sodium salt)
1004297-66-8 (free acid)
-
Absorption (max)
λmax = 295 nm
-
Ɛ (max)
9,500 cm-1M-1
-
Molecular Formula