C8-Alkyne-dUTP
Modified triphosphate for incorporation in PCR reaction
| Size | Catalog No. | Price |
|---|---|---|
| 1 µmol | BCT-05-S | € 100,00 |
| 5 µmol | BCT-05-L | € 300,00 |
Chemical Properties
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Molecular Formula
C17H23N2O14P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
572.29 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
100 mM clear colorless
solution in water (pH 7.5) -
CAS Number
1004297-65-7 (free acid)
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Absorption (max)
λmax = 292 nm
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Ɛ (max)
11.000 cm-1M-1
Product Information
C8-Alkyne-dUTP is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by click chemistry. We recommend a substitution of 1 – 10% of dUTP.
LITERATURE
Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry, J. Gierlich et al., 2007, Chem. – A Eur. J., Vol. 13, p. 9486–9494.
https://doi.org/10.1002/chem.200700502
Directed DNA Metallization, G. A. Burley et al., 2006, J. Am. Chem. Soc., Vol. 128, p. 1398–1399.
https://doi.org/10.1021/ja055517v
Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction, S. Hesse et al., 2016, Chromosome Research, Vol. 24(3), p. 299–307.
https://doi.org/10.1007/s10577-016-9522-z
Enzymatic Synthesis of Chemical Nuclease Triplex-Forming Oligonucleotides with Gene-Silencing Applications, B. McGorman et al., 2022, Nucleic Acids Research, Vol. 50(10), p. 5467–5481.